hElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.
hElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.
复制标题
DOI：
10.1371/journal.pone.0029303
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Han G
中科院分区：
文献类型：
--
作者：
Li F;Ma J;Ma Y;Hu Y;Tian S;White RE;Han G
人延伸子复合物在体外转录延长实验中起着关键作用，它与酵母延伸子复合物在成分和功能上都非常相似。然而，只有少数研究集中在其靶基因的体内表征。本研究通过反义策略下调人延伸蛋白3（human elongation protein 3，hELP3）对热休克蛋白70（HSP 70）表达的影响。反义质粒p1107转染HeLa细胞后，hELP3表达明显降低，HSP70 mRNA和蛋白表达明显降低。染色质免疫沉淀实验（ChIP Assay）显示，hElp3参与了HeLa细胞中HSPA1A的转录延伸。最后，在酵母中的互补和ChIP分析表明，hElp3不仅可以补充生长和减缓HSP 70（SSA3）基因转录的激活，而且可以直接调节SSA3的转录。相反，当HAT结构域从hElp3中缺失时，这些功能丧失。这些结果表明hElp3可以调控HSP 70基因的转录，而hElp3的HAT结构域是该功能所必需的。这些发现现在为hELP3在人类细胞中的功能提供了新的见解和证据。
Human Elongator complex, which plays a key role in transcript elongation in vitro assay, is incredibly similar in either components or function to its yeast counterpart. However, there are only a few studies focusing on its target gene characterization in vivo. We studied the effect of down-regulation of the human elongation protein 3 (hELP3) on the expression of HSP70 through antisense strategy. Transfecting antisense plasmid p1107 into HeLa cells highly suppressed hELP3 expression, and substantially reduced expression of HSP70 mRNA and protein. Furthermore, chromatin immunoprecipitation assay (ChIP Assay) revealed that hElp3 participates in the transcription elongation of HSPA1A in HeLa cells. Finally, complementation and ChIP Assay in yeast showed that hElp3 can not only complement the growth and slow activation of HSP70 (SSA3) gene transcription, but also directly regulates the transcription of SSA3. On the contrary, these functions are lost when the HAT domain is deleted from hElp3. These data suggest that hElp3 can regulate the transcription of HSP70 gene, and the HAT domain of hElp3 is essential for this function. These findings now provide novel insights and evidence of the functions of hELP3 in human cells.
登录
查看更多内容
影响因子：
4.5
作者：
Li Q;Fazly AM;Zhou H;Huang S;Zhang Z;Stillman B
通讯作者：
Stillman B
影响因子：
5.3
作者：
Giacconi, R;Caruso, C;Mocchegiani, E
通讯作者：
Mocchegiani, E
影响因子：
13.8
作者：
Chinenov, Y
通讯作者：
Chinenov, Y
影响因子：
16
作者：
Otero, G;Fellows, J;Svejstrup, JQ
通讯作者：
Svejstrup, JQ
影响因子：
3.6
作者：
Paraskevopoulou, C;Fairhurst, SA;Onesti, S
通讯作者：
Onesti, S