Effects of interleukin 12 on immune responses and host protection in mice infected with intestinal nematode parasites.
Effects of interleukin 12 on immune responses and host protection in mice infected with intestinal nematode parasites.
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白介素12对感染肠线虫寄生虫感染的小鼠免疫反应和宿主保护的影响。
DOI：
10.1084/jem.179.5.1563
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发表时间：
1994-05-01
期刊：
影响因子：
--
通讯作者：
Urban JF Jr
中科院分区：
文献类型：
--
作者：
Finkelman FD;Madden KB;Cheever AW;Katona IM;Morris SC;Gately MK;Hubbard BR;Gause WC;Urban JF Jr
细胞因子白细胞介素（IL）12刺激T细胞和自然杀伤细胞产生干扰素（IFN）γ，并抑制T细胞产生IL-4。我们研究了IL-12对细胞因子基因表达、免疫球蛋白（IG）E、粘膜肥大细胞和嗜酸性粒细胞反应的影响，以及接种线虫寄生虫巴西日本圆线虫的小鼠的感染过程，以及这些影响的IFN-γ依赖性。IL-12刺激IFN-γ和IL-10基因在原发性和继发性N.在初次感染过程中，对IL-3、IL-4、IL-5和IL-9基因表达有抑制作用，但在二次感染过程中几乎没有抑制作用。IL-12抑制IgE，粘膜肥大细胞，血液和组织中的嗜酸性粒细胞的反应在原发性感染，但只有嗜酸性粒细胞的反应在继发性感染。IL-12增强成虫的存活率和卵的产生在主要的，但不是继发感染。IL-12需要在初次感染的第4天施用以抑制IgE和粘膜肥大细胞反应，并且在第6天施用以强烈抑制嗜酸性粒细胞反应并增强蠕虫存活和繁殖力。抗IFN-γ mAb抑制IL-12对IgE分泌、肠粘膜肥大细胞增多症、寄生虫存活和繁殖力的影响，但不影响IL-12对嗜酸性粒细胞增多症的抑制。这些观察结果表明，如果在嗜酸性粒细胞增多症开始期间给予IL-12。这些观察结果表明，IL-12，如果在免疫应答的起始期间施用，可以将应答从以产生辅助性T细胞（Th）2相关细胞因子为特征的应答改变为以产生Th-1相关细胞因子为特征的应答。然而，一旦Th 2相关细胞因子的产生已经建立，IL-12治疗具有较小的效果。此外，我们的研究结果提供的证据表明，Th 2相关的反应保护，和/或Th 1相关的反应加剧，线虫感染。
The cytokine interleukin (IL) 12 stimulates T cell and natural killer cell production of interferon (IFN) gamma and inhibits T cell production of IL-4. We investigated the effects of IL-12 on cytokine gene expression, immunoglobulin (Ig)E, mucosal mast cell, and eosinophil responses, and the course of infection in mice inoculated with the nematode parasite Nippostrongylus brasiliensis, as well as the IFN-gamma dependence of these effects. IL-12 stimulated IFN-gamma and IL-10 gene expression during primary and secondary N. brasiliensis infections and inhibited IL-3, IL-4, IL-5, and IL-9 gene expression during primary infections but had little inhibitory effect during secondary infections. IL-12 inhibited IgE, mucosal mast cell, and blood and tissue eosinophil responses during primary infections, but only eosinophil responses during secondary infections. IL-12 enhanced adult worm survival and egg production during primary, but not secondary infections. IL-12 needed to be administered by day 4 of a primary infection to inhibit IgE and mucosal mast cell responses, and by day 6 to strongly inhibit eosinophil responses and to enhance worm survival and fecundity. Anti-IFN-gamma mAb inhibited the effects of IL-12 on IgE secretion, intestinal mucosal mastocytosis, and parasite survival and fecundity, but did not affect IL-12 inhibition of eosinophilia. These observations indicate that IL-12, if administered during the initiation of eosinophilia. These observations indicate that IL-12, if administered during the initiation of an immune response, can change the response from one that is characterized by the production of T helper (Th)2-associated cytokines to one characterized by the production of Th-1 associated cytokines. However, IL-12 treatment has less of an effect once the production of Th2-associated cytokines has become established. In addition, our results provide evidence that Th2- associated responses protect against, and/or Th1-associated responses exacerbate, nematode infections.
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重组白介素12治愈感染了利什曼原虫大调的小鼠。
影响因子：
15.3
作者：
Heinzel, F P;Schoenhaut, D S;Rerko, R M;Rosser, L E;Gately, M K
通讯作者：
Gately, M K
DOI：
10.1084/jem.179.4.1273
发表时间：
1994-04-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Manetti R;Gerosa F;Giudizi MG;Biagiotti R;Parronchi P;Piccinni MP;Sampognaro S;Maggi E;Romagnani S;Trinchieri G
通讯作者：
Trinchieri G
DOI：
10.1084/jem.171.5.1497
发表时间：
1990-05-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Conrad DH;Ben-Sasson SZ;Le Gros G;Finkelman FD;Paul WE
通讯作者：
Paul WE
影响因子：
15.9
作者：
KINIWA, M;GATELY, M;DELESPESSE, G
通讯作者：
DELESPESSE, G
DOI：
10.1084/jem.173.2.279
发表时间：
1991-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
HayGlass KT;Stefura BP
通讯作者：
Stefura BP