Elimination of the cold-chain dependence of a nanoemulsion adjuvanted vaccine against tuberculosis by lyophilization.
Elimination of the cold-chain dependence of a nanoemulsion adjuvanted vaccine against tuberculosis by lyophilization.
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DOI：
10.1016/j.jconrel.2013.12.025
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发表时间：
2014-03-10
期刊：
影响因子：
--
通讯作者：
Vedvick TS
中科院分区：
文献类型：
--
作者：
Orr MT;Kramer RM;Barnes L 5th;Dowling QM;Desbien AL;Beebe EA;Laurance JD;Fox CB;Reed SG;Coler RN;Vedvick TS
下一代合理设计的疫苗佐剂代表着一项重大突破，能够开发针对包括结核病、艾滋病毒和疟疾在内的挑战性疾病的疫苗。新的候选疫苗通常需要保持冷链过程以确保长期稳定，并需要分开的瓶子以使抗原和佐剂能够在床边混合。这对在全球范围内实施这类疫苗构成了重大的财政和技术障碍。在这里，我们描述了一种结核疫苗的开发和特性，该疫苗由抗原和佐剂成分组成，在持续高温下在单个小瓶中稳定。此外，该疫苗保留了诱导针对疫苗抗原的抗体和TH1反应的能力，并防止实验中对结核分枝杆菌的挑战。这些结果代表着候选疫苗开发方面的重大突破，这种疫苗可以在世界各地实施，而不会受到连续冷链或分开的佐剂和抗原瓶的必要性的阻碍。
Next-generation rationally-designed vaccine adjuvants represent a significant breakthrough to enable development of vaccines against challenging diseases including tuberculosis, HIV, and malaria. New vaccine candidates often require maintenance of a cold-chain process to ensure long-term stability and separate vials to enable bedside mixing of antigen and adjuvant. This presents a significant financial and technological barrier to worldwide implementation of such vaccines. Herein we describe the development and characterization of a tuberculosis vaccine comprised of both antigen and adjuvant components that are stable in a single vial at sustained elevated temperatures. Further this vaccine retains the ability to elicit both antibody and TH1 responses against the vaccine antigen and protect against experimental challenge with Mycobacterium tuberculosis. These results represent a significant breakthrough in the development of vaccine candidates that can be implemented throughout the world without being hampered by the necessity of a continuous cold chain or separate adjuvant and antigen vials.
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DOI：
10.1016/j.ejpb.2013.05.019
发表时间：
2013-10-01
影响因子：
4.9
作者：
Kasper, Julia Christina;Winter, Gerhard;Friess, Wolfgang
通讯作者：
Friess, Wolfgang
影响因子：
5.8
作者：
Anderson, Ryan C.;Fox, Christopher B.;Vedvick, Thomas S.
通讯作者：
Vedvick, Thomas S.
影响因子：
2.2
作者：
Ching, Lance K.;Mompoint, Farah;Baldwin, Susan L.
通讯作者：
Baldwin, Susan L.
影响因子：
64.8
作者：
Shiow, LR;Rosen, DB;Matloubian, M
通讯作者：
Matloubian, M
影响因子：
3.8
作者：
Chen, Dexiang;Zehrung, Darin
通讯作者：
Zehrung, Darin