Bacterial Type I Toxins: Folding and Membrane Interactions.
Bacterial Type I Toxins: Folding and Membrane Interactions.
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DOI：
10.3390/toxins13070490
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发表时间：
2021-07-14
期刊：
影响因子：
4.2
通讯作者：
Pinel-Marie ML
中科院分区：
文献类型：
--
作者：
Nonin-Lecomte S;Fermon L;Felden B;Pinel-Marie ML
细菌I型毒素-抗毒素系统是编码稳定的毒性蛋白和不稳定的RNA抗毒素的双组分遗传模块，所述稳定的毒性蛋白的异位过表达可导致生长停滞或细胞死亡，所述不稳定的RNA抗毒素在生长期间抑制毒素翻译。这些系统在细菌物种中广泛传播。I型抗毒素是顺式或反式编码的反义小RNA，其通过配对与毒素编码mRNA相互作用，从而抑制毒素mRNA翻译和/或诱导其降解。在环境胁迫条件下，通过特异性RNA酶上调毒素和/或抗毒素降解促进毒素翻译。大多数I型毒素是具有预测的α-螺旋跨膜结构域的小疏水肽，其诱导膜去极化和/或透化，随后细胞内ATP减少，导致质粒维持、对环境应激的生长适应或持续细胞形成。在这篇综述中，我们描述了目前的技术水平上的折叠和这些膜相关的I型毒素从革兰氏阴性或革兰氏阳性细菌的膜相互作用，并建立一个年表，其对细菌细胞的毒性作用。这篇综述还包括新的结构的结果，通过NMR有关的sprG 1编码的膜肽，属于sprG 1/SprF 1型TA系统在金黄色葡萄球菌中表达，并讨论了假定的膜相互作用，允许裂解的竞争细菌和宿主细胞。
Bacterial type I toxin-antitoxin systems are two-component genetic modules that encode a stable toxic protein whose ectopic overexpression can lead to growth arrest or cell death, and an unstable RNA antitoxin that inhibits toxin translation during growth. These systems are widely spread among bacterial species. Type I antitoxins are cis- or trans-encoded antisense small RNAs that interact with toxin-encoding mRNAs by pairing, thereby inhibiting toxin mRNA translation and/or inducing its degradation. Under environmental stress conditions, the up-regulation of the toxin and/or the antitoxin degradation by specific RNases promote toxin translation. Most type I toxins are small hydrophobic peptides with a predicted α-helical transmembrane domain that induces membrane depolarization and/or permeabilization followed by a decrease of intracellular ATP, leading to plasmid maintenance, growth adaptation to environmental stresses, or persister cell formation. In this review, we describe the current state of the art on the folding and the membrane interactions of these membrane-associated type I toxins from either Gram-negative or Gram-positive bacteria and establish a chronology of their toxic effects on the bacterial cell. This review also includes novel structural results obtained by NMR concerning the sprG1-encoded membrane peptides that belong to the sprG1/SprF1 type I TA system expressed in Staphylococcus aureus and discusses the putative membrane interactions allowing the lysis of competing bacteria and host cells.
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影响因子：
2.5
作者：
Berghoff BA;Wagner EGH
通讯作者：
Wagner EGH
影响因子：
14.9
作者：
Fozo EM;Makarova KS;Shabalina SA;Yutin N;Koonin EV;Storz G
通讯作者：
Storz G
影响因子：
3.6
作者：
Berghoff, Bork A.;Hoekzema, Mirthe;Wagner, E. Gerhart H.
通讯作者：
Wagner, E. Gerhart H.
影响因子：
3.2
作者：
Brinkman, Cassandra L.;Bumgarner, Roger;Weaver, Keith E.
通讯作者：
Weaver, Keith E.
影响因子：
11.4
作者：
GERDES, K;BECH, FW;VONMEYENBURG, K
通讯作者：
VONMEYENBURG, K