In Vitro Global Gene Expression Analyses Support the Ethnopharmacological Use of Achyranthes aspera.
In Vitro Global Gene Expression Analyses Support the Ethnopharmacological Use of Achyranthes aspera.
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DOI：
10.1155/2013/471739
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Ardalan B
中科院分区：
文献类型：
--
作者：
Subbarayan PR;Sarkar M;Nathanson L;Doshi N;Lokeshwar BL;Ardalan B
土牛膝（苋科）以其抗癌特性而闻名。我们已经系统地验证了这种植物的体外和体内抗癌特性。但是，我们不知道它的作用方式。全球基因表达分析可能有助于破译其作用模式。在没有确定的活性分子的情况下，我们相信这是发现具有已知药用特性的天然产物的作用模式的最佳方法。我们将人胰腺癌细胞系MiaPaCa-2（CRL-1420）暴露于34 μg/mL LE 24、48和72小时。使用全人类基因组微阵列（Agilent Technologies，USA）进行基因表达分析。在我们的分析中，82个（54/28）基因通过了质量控制参数，设定为FDR ≤ 0.01和FC ≥±2。LE主要影响免疫应答、代谢、发育、基因表达调节、细胞粘附、囊性纤维化跨膜传导调节（CFTR）和趋化性的途径（MetaCore工具（Thomson路透社，纽约））。疾病生物标志物富集分析鉴定了参与血管炎-血管炎症的LE调节基因。关节炎和胰腺炎是血管炎的两种病因。疾病网络分析的结果支持A.阿斯培拉，即止血，治疗胰腺癌，抗关节炎药物，等等。
Achyranthes aspera (family Amaranthaceae) is known for its anticancer properties. We have systematically validated the in vitro and in vivo anticancer properties of this plant. However, we do not know its mode of action. Global gene expression analyses may help decipher its mode of action. In the absence of identified active molecules, we believe this is the best approach to discover the mode of action of natural products with known medicinal properties. We exposed human pancreatic cancer cell line MiaPaCa-2 (CRL-1420) to 34 μg/mL of LE for 24, 48, and 72 hours. Gene expression analyses were performed using whole human genome microarrays (Agilent Technologies, USA). In our analyses, 82 (54/28) genes passed the quality control parameter, set at FDR ≤ 0.01 and FC of ≥±2. LE predominantly affected pathways of immune response, metabolism, development, gene expression regulation, cell adhesion, cystic fibrosis transmembrane conductance regulation (CFTR), and chemotaxis (MetaCore tool (Thomson Reuters, NY)). Disease biomarker enrichment analysis identified LE regulated genes involved in Vasculitis—inflammation of blood vessels. Arthritis and pancreatitis are two of many etiologies for vasculitis. The outcome of disease network analysis supports the medicinal use of A. aspera, viz, to stop bleeding, as a cure for pancreatic cancer, as an antiarthritic medication, and so forth.
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影响因子：
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作者：
Schroeder A;Mueller O;Stocker S;Salowsky R;Leiber M;Gassmann M;Lightfoot S;Menzel W;Granzow M;Ragg T
通讯作者：
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影响因子：
11.5
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通讯作者：
Soboleva A