Effects of UV radiation on optimum quantum yield and sunscreen contents in members of the genera Interfilum, Klebsormidium, Hormidiella and Entransia (Klebsormidiophyceae, Streptophyta)

Effects of UV radiation on optimum quantum yield and sunscreen contents in members of the genera Interfilum, Klebsormidium, Hormidiella and Entransia (Klebsormidiophyceae, Streptophyta)
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紫外线辐射对 Interfilum、Klebsormidium、Hormidiella 和 Entransia(Klebsormidiophyceae、Streptophyta)属成员的最佳量子产率和防晒剂含量的影响

DOI:
10.1080/09670262.2015.1031190
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发表时间:
2015
影响因子:
2.4
通讯作者:
Karsten
Karsten
中科院分区:
生物学3区
文献类型:
--
作者:
Kitzing;Karsten

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Klebsormidiophyceae的许多成员通常发生在陆地栖息地,在那里它们暴露于紫外线辐射(UVR)。为了评估对该波段的耐受程度,将32株entransia、Hormidiella、interfilumandklebsormidium属细菌暴露在相同的人工紫外线下。根据PSII的最佳量子产率以及合成和积累真菌孢素样氨基酸(MAAs)作为防晒霜的能力来确定各自的响应模式。暴露于UVR后,大多数被调查的hormidiella菌株,interfilumandklebsormidium的最佳量子产率只有中等或没有下降。相反,两种菌株都表现出强烈的光抑制作用。这些光合活性数据很好地反映在合成MAAs的生化能力上。除了这两种过渡性菌株甚至没有检测到MAAs的痕迹外,所有其他的hormidiella, interfilumanand klebsormidia菌株在紫外线照射后都积累了吸收紫外线的化合物。虽然interfilumandklebsormidi含有相同的MAA,在324 nm处吸收最大值,但Hormidiella衰减显示另一种MAA,在325 nm处吸收最大值。此外,两种化合物在HPLC分析中表现出不同的保留时间,表明它们的化学结构一定不同。所有数据都表明,hormidiella, interfilumandklebsormidia具有普遍较高的UVR耐受性,这很好地解释了它们的空陆生活方式。
Many members of the Klebsormidiophyceae typically occur in terrestrial habitats where they are exposed to ultraviolet radiation (UVR). To evaluate the extent of tolerance to this waveband, 32 strains of the generaEntransia,Hormidiella,InterfilumandKlebsormidiumwere exposed to identical artificial UVR. The respective response patterns were determined in terms of optimum quantum yield of PSII as well as the capability to synthesize and accumulate mycosporine-like amino acids (MAAs) as sunscreens. After exposure to UVR most of the investigated isolates ofHormidiella,InterfilumandKlebsormidiumexhibited only a moderate or no decrease in optimum quantum yield. In contrast, both isolates ofEntransiashowed strong photoinhibition. These photosynthetic activity data are well reflected in the biochemical capability to synthesize MAAs. With the exception of bothEntransiastrains, where not even traces of MAAs could be detected, all other isolates ofHormidiella,InterfilumandKlebsormidiumaccumulated UV-absorbing compounds after UVR exposure. WhileInterfilumandKlebsormidiumcontained the same putative MAA with an absorption maximum at 324 nm,Hormidiella attenuatashowed another putative MAA with an absorption maximum at 325 nm. In addition, both compounds exhibited different retention times during HPLC analysis, suggesting their chemical structure must be different. All data indicated a generally high UVR tolerance inHormidiella,InterfilumandKlebsormidiumwhich explains well their aeroterrestrial lifestyle.
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