Cloning of the β2a Subunit of the Voltage-Dependent Calcium Channel from Human Heart: Cooperative Effect of α2/δ and β2a on the Membrane Expression of the α1C Subunit ☆ ☆☆

Cloning of the β2a Subunit of the Voltage-Dependent Calcium Channel from Human Heart: Cooperative Effect of α2/δ and β2a on the Membrane Expression of the α1C Subunit ☆ ☆☆
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人心脏电压依赖性钙通道β2a亚基的克隆：α2/δ和β2a对α1C亚基膜表达的协同作用☆☆☆

DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
G. Varadi
G. Varadi
中科院分区：
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文献类型：
--
作者：
H. Yamaguchi;M. Okuda;G. Mikala;K. Fukasawa;G. Varadi

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通过共聚焦显微镜和电生理记录监测爪蟾卵母细胞中表位标记的人Ca（2+）通道α（1C）亚基的表达和膜定位。当α（2）/δ和β（2a）分别与α（1C）亚基共表达时，通过共聚焦显微镜评估显示通道密度分别增加了86%和225%。α（2）/δ和β（2a）亚基的同时共表达导致协同（470%）增加。平行进行的电生理学测量显示，α（2）/δ亚基的电流增加完全归因于通道密度的增加，而β（2a）亚基除了增加通道密度外，还促进通道开放。
Expression and membrane localization of an epitope-tagged human Ca(2+) channel alpha(1C) subunit were monitored in Xenopus oocytes by confocal microscopy and electrophysiological recording. When alpha(2)/delta and beta(2a) were separately coexpressed with the alpha(1C) subunit, assessment by confocal microscopy showed an 86 and 225% increase of the channel density, respectively. Simultaneous coexpression of alpha(2)/delta and beta(2a) subunits resulted in a cooperative (470%) increase. Electrophysiological measurements performed in parallel revealed that the current augmentation by the alpha(2)/delta subunit is totally attributable to an increase in channel density, whereas the beta(2a) subunit, in addition to increasing channel density, also facilitates channel opening.
Ca2+ current enhancement by alpha 2/delta and beta subunits in Xenopus oocytes: contribution of changes in channel gating and alpha 1 protein level.
非洲爪蟾卵母细胞中 α2/δ 和 β 亚基对 Ca2 电流的增强：通道门控和 α1 蛋白水平变化的贡献。
DOI： 10.1113/jphysiol.1995.sp021029
发表时间： 1995
期刊： The Journal of physiology
影响因子： --
作者：
Shistik,E;Ivanina,T;Puri,T;Hosey,M;Dascal,N
通讯作者： Dascal,N
The beta subunit increases Ca2+ currents and gating charge movements of human cardiac L-type Ca2+ channels.
β 亚基可增加 Ca2 电流并控制人类心脏 L 型 Ca2 通道的电荷运动。
DOI： 10.1016/s0006-3495(96)79685-6
发表时间： 1996
期刊： Biophysical journal.
影响因子： --
作者：
Josephson,IR;Varadi,G
通讯作者： Varadi,G
Critical role of conserved proline residues in the transmembrane segment 4 voltage sensor function and in the gating of L-type calcium channels.
保守脯氨酸残基在跨膜段 4 电压传感器功能和 L 型钙通道门控中发挥关键作用。
DOI： 10.1073/pnas.96.4.1357
发表时间： 1999
期刊： Proceedings of the National Academy of Sciences of the United States of America
影响因子： 11.1
作者：
Yamaguchi,H;Muth,JN;Varadi,M;Schwartz,A;Varadi,G
通讯作者： Varadi,G
Unique regulatory properties of the type 2a Ca2+ channel β subunit caused by palmitoylation
DOI： 10.1073/pnas.95.8.4690
发表时间： 1998-04-14
期刊： PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子： 11.1
作者：
Qin, N;Platano, D;Birnbaumer, L
通讯作者： Birnbaumer, L
Cloning, chromosomal localization, and functional expression of the alpha 1 subunit of the L-type voltage-dependent calcium channel from normal human heart.
DOI： 10.1073/pnas.90.13.6228
发表时间： 1993-07
期刊： Proceedings of the National Academy of Sciences of the United States of America
影响因子： 11.1
作者：
David Schultz;Gabor Mikala;Atsuko Yatani;Dorothy B. Engle;David E. ILESt;Bart SEGERSt;Richard J. Sinke;D. Weghuis;Udo Klockner;Minoru Wakamori;JING-JING Wang-JING;David Melvin;G. Varadi;Arnold Schwartz
通讯作者： David Schultz;Gabor Mikala;Atsuko Yatani;Dorothy B. Engle;David E. ILESt;Bart SEGERSt;Richard J. Sinke;D. Weghuis;Udo Klockner;Minoru Wakamori;JING-JING Wang-JING;David Melvin;G. Varadi;Arnold Schwartz