Clinical Value of Combined Determination of Serum B7-H4 with Carcinoembryonic Antigen, Osteopontin, or Tissue Polypeptide-Specific Antigen for the Diagnosis of Colorectal Cancer.
Clinical Value of Combined Determination of Serum B7-H4 with Carcinoembryonic Antigen, Osteopontin, or Tissue Polypeptide-Specific Antigen for the Diagnosis of Colorectal Cancer.
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DOI：
10.1155/2018/4310790
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Gai X
中科院分区：
文献类型：
--
作者：
Wang P;Li C;Zhang F;Ma X;Gai X
B7-H4是B7家族的成员，负调节免疫应答，这与肿瘤的发展和预后相关。本研究旨在检测大肠癌患者血清中B7-H4的表达，并探讨其在大肠癌诊断中的作用。 我们检测了59例大肠癌患者和29例健康志愿者血清中B7-H4、癌胚抗原（CEA）、骨桥蛋白（OPN）和组织多肽特异性抗原（TPS）的表达，分析了B7-H4联合CEA、OPN和TPS检测对大肠癌的诊断价值。采用酶联免疫吸附法检测血清B7-H4、OPN和TPS的表达，采用电化学发光法检测CEA。 结直肠癌患者血清B7-H4水平显著高于配对正常对照组（P = 0.001）。血清B7-H4水平与肿瘤浸润深度、肿块大小和淋巴结转移呈正相关（分别为P = 0.004、P = 0.016和P = 0.0052）。我们还检测了根治性切除术前后血清中B7-H4的表达，发现B7-H4水平在术后第一周显著下降（P = 0.0064）。我们使用受试者工作特征（ROC）曲线分析，以表明这些标志物的潜在诊断价值。B7-H4、OPN、TPS和CEA的ROC曲线下面积（AUC）分别为0.867、0.805、0.812和0.833。B7-H4用于区分结肠癌患者和健康对照的最佳灵敏度和特异性分别为88.2%和86.7%，使用78.89 ng/mL的截断值。然而，使用B7-H4和CEA的组合ROC分析显示AUC为0.929，区分结肠癌患者和健康对照的灵敏度为98.9%，特异性为80.4%。 B7-H4在大肠癌患者血清中呈高表达。与单独检测这些标志物相比，检测B7-H4加CEA显示用于区分结直肠癌患者和健康对照的灵敏度和特异性显著增加。联合检测血清B7-H4和CEA有可能成为结直肠癌早期临床诊断和预后评估的一种新的实验室方法。
B7-H4 is member of the B7 family that negatively regulates the immune response, which are associated with tumor development and prognosis. The present study is aimed at examining serum B7-H4 expression and exploring its contribution to diagnosis in patients with colorectal cancer. We determined serum expressions of B7-H4, carcinoembryonic antigen (CEA), osteopontin (OPN), and tissue polypeptide-specific antigen (TPS) in 59 patients with colorectal cancer and 29 healthy volunteers and analyzed the diagnostic value of B7-H4 combined with CEA, OPN, or TPS detection for colorectal cancer. B7-H4, OPN, and TPS serum expressions were measured by enzyme-linked immunosorbent assay, and CEA was measured by electrochemical luminescence detection. Serum B7-H4 levels were significantly higher in colorectal cancer patients compared with paired normal controls (P = 0.001). B7-H4 serum level was positively correlated with infiltration depth, tumor masses, and lymph node metastasis (P = 0.004, P = 0.016, and P = 0.0052, respectively). We also detected serum expression of B7-H4 before and after radical resection and showed that B7-H4 levels decreased significantly during the first week postoperation (P = 0.0064). We used receiver operating characteristic (ROC) curve analysis to indicate the potential diagnostic values of these markers. The areas under the ROC curves (AUC) for B7-H4, OPN, TPS, and CEA were 0.867, 0.805, 0.812, and 0.833, respectively. The optimal sensitivity and specificity of B7-H4 for discriminating between colon cancer patients and healthy controls were 88.2% and 86.7%, respectively, using a cut-off of value of 78.89 ng/mL. However, combined ROC analysis using B7-H4 and CEA revealed an AUC of 0.929, with a sensitivity of 98.9% and a specificity of 80.4% for discriminating colon cancer patients from healthy controls. B7-H4 was highly expressed in the serum in colorectal cancer patients. Detection of B7-H4 plus CEA showed significantly increased sensitivity and specificity for discriminating between colorectal cancer patients and healthy controls compared to individual detection of these markers. Combined detection of serum B7-H4 and CEA may thus have the potential to become a new laboratory method for the early clinical diagnosis and prognostic evaluation of colorectal cancer.
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DOI：
10.1093/annonc/mdy085
发表时间：
2018-05-01
期刊：
Annals of oncology : official journal of the European Society for Medical Oncology
影响因子：
--
作者：
Smeby J;Sveen A;Merok MA;Danielsen SA;Eilertsen IA;Guren MG;Dienstmann R;Nesbakken A;Lothe RA
通讯作者：
Lothe RA
血清中 microRNA 的表征：一类用于诊断癌症和其他疾病的新型生物标志物
影响因子：
44.1
作者：
Chen, Xi;Ba, Yi;Zhang, Chen-Yu
通讯作者：
Zhang, Chen-Yu
血清B7-H4表达是胃癌患者重要的预后指标
影响因子：
3.2
作者：
Shi, Hongbing;Ji, Mei;Jiang, Jingting
通讯作者：
Jiang, Jingting
影响因子：
3.8
作者：
Holmer R;Wätzig GH;Tiwari S;Rose-John S;Kalthoff H
通讯作者：
Kalthoff H
血浆 miR-601 和 miR-760 是早期检测结直肠癌的新型生物标志物
影响因子：
3.7
作者：
Wang Q;Huang Z;Ni S;Xiao X;Xu Q;Wang L;Huang D;Tan C;Sheng W;Du X
通讯作者：
Du X