Freeze-thaw treatment effects on the dynamic mechanical properties of articular cartilage.
Freeze-thaw treatment effects on the dynamic mechanical properties of articular cartilage.
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DOI：
10.1186/1471-2474-11-231
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发表时间：
2010-10-08
影响因子：
2.3
通讯作者：
Bertram JE
中科院分区：
文献类型：
--
作者：
Szarko M;Muldrew K;Bertram JE
作为一种相对非再生的组织，关节软骨已被靶向用于冷冻保存，作为减轻移植手术供体组织可用性缺乏的方法。此外，关节软骨的零度以下储存长期以来一直用于使用各种储存温度的生物医学研究。目前的调查研究的潜在冻融影响关节软骨的机械性能，通过直接比较各种零下储存温度。使用对照样品（4°C）和储存在-20 ° C或-80 ° C下的样品以及在储存在-80 ° C下之前首先在液氮（_196 ° C）中快速冷冻的样品比较零度以下储存温度以及冷冻速率。将所有样品在37.5°C下解冻至测试温度（22°C）。复杂的刚度和滞后特性的负载电阻和阻尼特性，使用非破坏性的，低的力大小，动态压痕协议跨越广泛的加载速率范围，以确定动态粘弹性特性的软骨。刚度水平保持不变，暴露于各种零度以下的温度。在-196 ° C速冻并储存在-80 ° C的样品中，滞后增加，但暴露于其他储存温度时保持不变。所示的机械变化可能是由于冰透镜的形成，其中，冻胀效应可能导致胶原蛋白损伤。储存到-20 ° C和-80 ° C并没有改变关节软骨的机械特性，这表明当与快速解冻方案结合到37.5°C时，组织可以成功地储存在零度以下的温度下。
As a relatively non-regenerative tissue, articular cartilage has been targeted for cryopreservation as a method of mitigating a lack of donor tissue availability for transplant surgeries. In addition, subzero storage of articular cartilage has long been used in biomedical studies using various storage temperatures. The current investigation studies the potential for freeze-thaw to affect the mechanical properties of articular cartilage through direct comparison of various subzero storage temperatures. Both subzero storage temperature as well as freezing rate were compared using control samples (4°C) and samples stored at either -20°C or -80°C as well as samples first snap frozen in liquid nitrogen (-196°C) prior to storage at -80°C. All samples were thawed at 37.5°C to testing temperature (22°C). Complex stiffness and hysteresis characterized load resistance and damping properties using a non-destructive, low force magnitude, dynamic indentation protocol spanning a broad loading rate range to identify the dynamic viscoelastic properties of cartilage. Stiffness levels remained unchanged with exposure to the various subzero temperatures. Hysteresis increased in samples snap frozen at -196°C and stored at -80°C, though remained unchanged with exposure to the other storage temperatures. Mechanical changes shown are likely due to ice lens creation, where frost heave effects may have caused collagen damage. That storage to -20°C and -80°C did not alter the mechanical properties of articular cartilage shows that when combined with a rapid thawing protocol to 37.5°C, the tissue may successfully be stored at subzero temperatures.
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影响因子：
2.4
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