Mixed-polarization phenotype of ascites-associated macrophages in human ovarian carcinoma: correlation of CD163 expression, cytokine levels and early relapse.
Mixed-polarization phenotype of ascites-associated macrophages in human ovarian carcinoma: correlation of CD163 expression, cytokine levels and early relapse.
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DOI：
10.1002/ijc.28335
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发表时间：
2014-01-01
影响因子：
6.4
通讯作者：
Mueller, Rolf
中科院分区：
文献类型：
--
作者：
Reinartz, Silke;Schumann, Tim;Finkernagel, Florian;Wortmann, Annika;Jansen, Julia M.;Meissner, Wolfgang;Krause, Michael;Schwoerer, Anne-Marie;Wagner, Uwe;Mueller-Bruesselbach, Sabine;Mueller, Rolf
卵巢癌通常伴有含有大量巨噬细胞的恶性腹水的发生。研究表明，这些肿瘤相关巨噬细胞（tam）偏向于替代极化（M2），因此在治疗抵抗和转移扩散中起重要作用。在我们的研究中，我们探讨了浆液性卵巢癌中TAM极化的性质、调控及其临床相关性。在一项包含20例可评估患者的前瞻性研究中，分析了30例患者的TAMs和腹水细胞因子水平上的巨噬细胞极化标记物，并与无复发生存（RFS）相关。M2标记CD163在tam表面的表达与RFS呈负相关（p < 0.01）。然而，其中17例患者的全球基因表达谱显示出与M1/M2分类无关的混合极化表型。CD163表面表达与腹水IL-6、IL-10水平相关（p < 0.05），两种细胞因子均诱导CD163表达，其腹水水平与RFS呈明显负相关（p < 0.01）。这些发现定义了一个高CD163表达、高IL-6和/或IL-10水平和临床预后差的患者亚组。
Ovarian cancer is typically accompanied by the occurrence of malignant ascites containing large number of macrophages. It has been suggested that these tumor-associated macrophages (TAMs) are skewed to alternative polarization (M2) and thereby play an essential role in therapy resistance and metastatic spread. In our study, we have investigated the nature, regulation and clinical correlations of TAM polarization in serous ovarian cancer. Macrophage polarization markers on TAMs and ascites cytokine levels were analyzed for 30 patients and associated with relapse-free survival (RFS) in a prospective study with 20 evaluable patients. Surface expression of the M2 marker CD163 on TAMs was inversely associated with RFS (p < 0.01). However, global gene expression profiles determined for 17 of these patients revealed a mixed-polarization phenotype unrelated to the M1/M2 classification. CD163 surface expression also correlated with the ascites levels of IL-6 and IL-10 (p < 0.05), both cytokines induced CD163 expression, and their ascites levels showed a clear inverse association with RFS (p < 0.01). These findings define a subgroup of patients with high CD163 expression, high IL-6 and/or IL-10 levels and poor clinical outcome.
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影响因子：
3.8
作者：
Lane D;Matte I;Rancourt C;Piché A
通讯作者：
Piché A
影响因子：
2.8
作者：
Lan, Chunyan;Huang, Xin;Liu, Jihong
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Liu, Jihong
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Ghassabeh, Gholamreza Hassanzadeh;De Baetselier, Patrick;Raes, Geert
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Raes, Geert
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Biswas, SK;Gangi, L;Sica, A
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Sica, A
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20.3
作者：
Duluc, Dorothee;Delneste, Yves;Jeannin, Pascale
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Jeannin, Pascale