MaAts, an Alkylsulfatase, Contributes to Fungal Tolerances against UV-B Irradiation and Heat-Shock in Metarhizium acridum.
MaAts, an Alkylsulfatase, Contributes to Fungal Tolerances against UV-B Irradiation and Heat-Shock in Metarhizium acridum.
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DOI：
10.3390/jof8030270
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发表时间：
2022-03-08
期刊：
影响因子：
--
通讯作者：
Xia Y
中科院分区：
文献类型：
--
作者：
Song L;Xue X;Wang S;Li J;Jin K;Xia Y
磺化酶通常分为三类：I型、II型和III型磺化酶。III型硫酸酯酶烷基硫酸酯酶能水解十二烷基硫酸钠和辛基硫酸钠等伯烷基硫酸盐。因此，SDS生物降解具有潜在的应用前景。然而，烷基硫酸酯酶在生物防治真菌中的作用尚不清楚。本研究从绿僵菌（Metarhizium acridum）中鉴定出一个烷基硫酸酯酶基因MaAts。构建缺失菌株（ΔMaAts）和补充菌株（CP），以揭示其在M. acridum中的功能。与野生型（WT）相比，ΔMaAts中烷基硫酸酯酶活性显著降低。MaAts的丢失延迟了分生孢子的萌发和分生，并显著降低了真菌对UV-B照射和热休克的耐受性，而真菌的分生孢子产量和毒力未受影响。maat失活后，抗逆性相关基因的转录水平发生了显著变化。此外，数字基因表达谱显示，鉴定出512个差异表达基因（deg），其中ΔMaAts中上调基因177个，下调基因335个。在这些deg中，一些基因参与黑色素合成、细胞壁完整性和对各种胁迫的耐受性。这些结果表明，参与真菌耐胁迫的maat和deg可能是提高真菌农药耐胁迫性的候选基因。
Sulfatases are commonly divided into three classes: type I, type II, and type III sulfatases. The type III sulfatase, alkylsulfatase, could hydrolyze the primary alkyl sulfates, such as sodium dodecyl sulfate (SDS) and sodium octyl sulfate. Thus, it has the potential application of SDS biodegradation. However, the roles of alkylsulfatase in biological control fungus remain unclear. In this study, an alkylsulfatase gene MaAts was identified from Metarhizium acridum. The deletion strain (ΔMaAts) and the complemented strain (CP) were constructed to reveal their functions in M. acridum. The activity of alkylsulfatase in ΔMaAts was dramatically reduced compared to the wild-type (WT) strain. The loss of MaAts delayed conidial germination, conidiation, and significantly declined the fungal tolerances to UV-B irradiation and heat-shock, while the fungal conidial yield and virulence were unaffected in M. acridum. The transcription levels of stress resistance-related genes were significantly changed after MaAts inactivation. Furthermore, digital gene expression profiling showed that 512 differential expression genes (DEGs), including 177 up-regulated genes and 335 down-regulated genes in ΔMaAts, were identified. Of these DEGs, some genes were involved in melanin synthesis, cell wall integrity, and tolerances to various stresses. These results indicate that MaAts and the DEGs involved in fungal stress tolerances may be candidate genes to be adopted to improve the stress tolerances of mycopesticides.
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DOI：
10.1016/j.bbrc.2017.07.092
发表时间：
2017-09-16
影响因子：
3.1
作者：
Waddell GL;Gilmer CR;Taylor NG;Reveral JRS;Forconi M;Fox JL
通讯作者：
Fox JL
影响因子：
3.9
作者：
Agrawal S;Ransom RF;Saraswathi S;Garcia-Gonzalo E;Webb A;Fernandez-Martinez JL;Popovic M;Guess AJ;Kloczkowski A;Benndorf R;Sadee W;Smoyer WE;On Behalf Of The Pediatric Nephrology Research Consortium Pnrc
通讯作者：
On Behalf Of The Pediatric Nephrology Research Consortium Pnrc
影响因子：
4.2
作者：
Adang LA;Schlotawa L;Groeschel S;Kehrer C;Harzer K;Staretz-Chacham O;Silva TO;Schwartz IVD;Gärtner J;De Castro M;Costin C;Montgomery EF;Dierks T;Radhakrishnan K;Ahrens-Nicklas RC
通讯作者：
Ahrens-Nicklas RC
影响因子：
4.5
作者：
Gao Q;Jin K;Ying SH;Zhang Y;Xiao G;Shang Y;Duan Z;Hu X;Xie XQ;Zhou G;Peng G;Luo Z;Huang W;Wang B;Fang W;Wang S;Zhong Y;Ma LJ;St Leger RJ;Zhao GP;Pei Y;Feng MG;Xia Y;Wang C
通讯作者：
Wang C
影响因子：
4.9
作者：
Hayashi, Shimpei;Ishii, Tadashi;Hirayama, Takashi
通讯作者：
Hirayama, Takashi