Cloning and sequencing of rabbit leukocyte NADPH oxidase genes reveals a unique p67phox homolog
Cloning and sequencing of rabbit leukocyte NADPH oxidase genes reveals a unique p67phox homolog
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兔白细胞 NADPH 氧化酶基因的克隆和测序揭示了独特的 p67phox 同源物
DOI：
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发表时间：
2002
影响因子：
5.5
通讯作者：
M. Quinn
中科院分区：
文献类型：
--
作者：
Katherine A. Gauss;P. Mascolo;D. Siemsen;L. K. Nelson;P. L. Bunger;P. Pagano;M. Quinn
NADPH氧化酶在免疫和非免疫细胞功能中起重要作用。因为兔子代表了一个既定的模型，用于研究一些重要的疾病过程，涉及NADPH氧化酶的活性，我们进行了研究，克隆和序列的所有五个兔白细胞NADPH氧化酶基因。与其他物种的序列比较表明，除了p67 phox，兔phox蛋白是高度保守的。相比之下，兔p67 phox具有非常不同的C末端，并且比任何其他已知的p67 phox同源物长17个氨基酸。这是令人惊讶的，因为之前测序的所有phox蛋白质都高度保守。为了评价这种差异的功能后果，表达野生型兔p67 phox和缺失C末端17个氨基酸的突变兔p67 phox，并在无细胞测定中进行分析。我们的结果表明，全长和截短的兔p67 phox蛋白能够支持氧化酶活性，尽管截短形式可重复地支持比全长p67 phox更高水平的活性。这些研究有助于我们了解不同物种白细胞NADPH氧化酶的性质，并将在未来的研究中使用兔模型的价值。
The NADPH oxidase plays an important role in immune and nonimmune cell functions. Because rabbits represent an established model for studying a number of important disease processes that involve NADPH oxidase activity, we carried out studies to clone and sequence all five rabbit leukocyte NADPH oxidase genes. Comparison of the rabbit sequences with those of other species showed that, with the exception of p67phox, the rabbit phox proteins were highly conserved. In contrast, rabbit p67phox had a very divergent C‐terminus and was 17 amino acids longer than any other known p67phox homolog. This was surprising, given the high degree of conservation among all of the phox proteins sequenced previously. To evaluate the functional consequences of this difference, wild‐type rabbit p67phox and a mutated rabbit p67phox missing the C‐terminal 17 amino acids were expressed and analyzed in a cell‐free assay. Our results show that the full‐length and truncated rabbit p67phox proteins were able to support oxidase activity, although the truncated form reproducibly supported a higher level of activity than full‐length p67phox. These studies contribute to our understanding of the nature of the leukocyte NADPH oxidase in different species and will be valuable in future research using the rabbit model.
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bcr 缺失突变体中中性粒细胞呼吸爆发增加。
影响因子：
64.5
作者：
Voncken,JW;vanSchaick,H;Kaartinen,V;Deemer,K;Coates,T;Landing,B;Pattengale,P;Dorseuil,O;Bokoch,GM;Groffen,J
通讯作者：
Groffen,J
DOI：
--
发表时间：
1994-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Benna;L. R. Faust;B. Babior
通讯作者：
J. Benna;L. R. Faust;B. Babior
影响因子：
15.9
作者：
DINAUER, MC;PIERCE, EA;ORKIN, SH
通讯作者：
ORKIN, SH
影响因子：
6.4
作者：
Clark, RA
通讯作者：
Clark, RA
影响因子：
15.9
作者：
FAUST, LP;ELBENNA, J;CHANOCK, SJ
通讯作者：
CHANOCK, SJ