Rapid selection and identification of Miscanthus genotypes with enhanced glucan and xylan yields from hydrothermal pretreatment followed by enzymatic hydrolysis.
Rapid selection and identification of Miscanthus genotypes with enhanced glucan and xylan yields from hydrothermal pretreatment followed by enzymatic hydrolysis.
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DOI：
10.1186/1754-6834-5-56
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发表时间：
2012-08-03
影响因子：
6.3
通讯作者：
Yang B
中科院分区：
文献类型：
--
作者：
Zhang T;Wyman CE;Jakob K;Yang B
由于许多芒属植物可以培养出相对较高的生产力和碳水化合物含量，因此芒属植物作为一种能源作物具有很大的潜力，可以支持大规模的生物燃料生产。在本研究中，采用间歇水热预处理，温度为180℃，时间为35 ，然后酶解，可获得最高的芒属植物总糖得率。伊利诺伊州种植在伊利诺伊州。在180℃、35 m in和17.5 m in的高通量预处理，然后在多孔间歇反应器中进行共水解，在80个品种中，有两个品种的前处理和酶水解的产糖率显著高于其他品种。然后，表现上的差异与80个品种的组成有关，以提供对芒属植物提高产糖量的可取性状的洞察。高通量前处理和共水解法(HTPH)快速从多种芒属基因中鉴定出有前景的基因类型，包括芒属甘蔗/中华芒属和芒属的杂交种，从而将更具商业前景的物种与其他物种区分开来。芒果中总葡聚糖和木聚糖的含量对产量和理论产量都有影响，而木质素和灰分含量对生产性能没有可预测的影响。
Because many Miscanthus genotypes can be cultivated with relatively high productivity and carbohydrate content, Miscanthus has great potential as an energy crop that can support large scale biological production of biofuels. In this study, batch hydrothermal pretreatment at 180°C for 35 min followed by enzymatic hydrolysis was shown to give the highest total sugar yields for Miscanthus x giganteus cv. Illinois planted in Illinois. High throughput pretreatment at 180°C for 35 min and 17.5 min followed by co-hydrolysis in a multi-well batch reactor identified two varieties out of 80 that had significantly higher sugar yields from pretreatment and enzymatic hydrolysis than others. The differences in performance were then related to compositions of the 80 varieties to provide insights into desirable traits for Miscanthus that enhance sugar yields. High throughput pretreatment and co-hydrolysis (HTPH) rapidly identified promising genotypes from a wide range of Miscanthus genotypes, including hybrids of Miscanthus sacchariflorus/M. sinensis and Miscanthus lutarioriparius, differentiating the more commercially promising species from the rest. The total glucan plus xylan content in Miscanthus appeared to influence both mass and theoretical yields, while lignin and ash contents did not have a predictable influence on performance.
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影响因子：
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