Glutathione pool size affects cell survival after hyperthermic treatment.
Glutathione pool size affects cell survival after hyperthermic treatment.
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谷胱甘肽库的大小影响高温治疗后的细胞存活率。
DOI：
10.1007/bf00120165
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发表时间：
1985
影响因子：
6.1
通讯作者：
Meredith,MJ
中科院分区：
文献类型：
--
作者：
Freeman,ML;Malcolm,AW;Meredith,MJ
通过暴露于不同浓度的马来酸二乙酯（DEM），测定了中国仓鼠卵巢细胞内谷胱甘肽（GSH）的浓度。所获得的谷胱甘肽的各种稳态水平在整个实验时间过程中保持不变。加入DEM后，细胞在42°孵育，以集落形成为终点，得到热剂量响应曲线。随后测定剂量响应曲线的斜率，并与细胞内谷胱甘肽浓度进行比较。这一比较表明，中国仓鼠卵巢细胞含有多个谷胱甘肽储存库，而谷胱甘肽储存库又以逐步的方式调节热毒性。去除50%或更少的谷胱甘肽不影响热敏性。当50%至80%的谷胱甘肽被去除时，敏感性略有增加。去除超过80%的谷胱甘肽会显著增加热毒性。10µM和20µM DEM会产生广泛的谷胱甘肽耗竭，而存活率只有很小的变化，这意味着在热毒性受到影响之前，必须去除谷胱甘肽的阈值浓度。
Intracellular glutathione (GSH) concentrations were titrated in Chinese hamster ovary cells by exposure to various concentrations of diethylmaleate (DEM). The various steady state levels of GSH obtained were maintained throughout the experimental time course. Cells were incubated at 42° after DEM addition in order to produce thermal dose response curves using colony formation as the end point. The slope of the dose response curve was subsequently determined and compared to the intracellular GSH concentration. This comparison indicated Chinese hamster ovary cells contain multiple reservoirs of GSH which in turn regulate thermal toxicity in a stepwise manner. Removal of 50% or less of the GSH did not affect thermal sensitivity. A small increase in sensitivity occured when 50 to 80% of the GSH was removed. Removal of greater than 80% of the GSH increased thermal toxicity significantly. The facts that 10 and 20 µM DEM produce extensive GSH depletion and only small changes in survival imply that a threshold concentration of GSH must be removed before thermal toxicity is affected.
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影响因子：
8.8
作者：
Russo A;Mitchell JB;McPherson S
通讯作者：
McPherson S
丁硫氨酸亚磺酰亚胺减少细胞谷胱甘肽以及对 L-苯丙氨酸芥子耐药的小鼠肿瘤细胞的敏化。
影响因子：
5.8
作者：
S. Somfai;K. Suzukake;B. Vistica;D. Vistica
通讯作者：
D. Vistica
影响因子：
2.9
作者：
REED, DJ;BABSON, JR;POTTER, DW
通讯作者：
POTTER, DW
马来酸二乙酯对离体肝细胞蛋白质合成的影响。
影响因子：
4.5
作者：
F. Goethals;G. Krack;D. Deboyser;M. Roberfroid
通讯作者：
M. Roberfroid
提出的基于营养物质和初始处理温度的影响的耐热性操作模型。
影响因子：
11.2
作者：
Li,GC;Hahn,GM
通讯作者：
Hahn,GM