Effect of collagen digestion on the passive elastic properties of diaphragm muscle in rat.
Effect of collagen digestion on the passive elastic properties of diaphragm muscle in rat.
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DOI：
10.1016/j.medengphy.2009.11.002
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发表时间：
2010-01
影响因子：
2.2
通讯作者：
An, Kai-Nan
中科院分区：
文献类型：
--
作者：
Rowe, Justin;Chen, Qingshan;Domire, Zachary J.;McCullough, Matthew B.;Sieck, Gary;Zhan, Wen-Zhi;An, Kai-Nan
胶原蛋白消化的影响已被定义到原纤维水平。然而，骨骼肌细胞外基质（ECM）的改变是否会影响肌肉的被动弹性反应仍然是一个问题。各种弹性成像方法已被用作评估骨骼肌的机械性能和 ECM 含量的工具。为了开发 ECM 改变的骨骼肌模型，本研究确定了胶原蛋白消化对骨骼肌被动弹性特性的影响。在不同程度的胶原消化下评估大鼠隔膜的被动机械性能。在循环负荷测试之间，将肌肉条浸入不同浓度的溶组织梭菌衍生的细菌胶原酶中。随后通过光学显微镜观察所有样品。循环测试揭示了多个浓度下被动肌肉硬度和消化时间之间的线性关系。这些结果表明，骨骼肌中 ECM 的胶原酶消化可用作机械改变的体外组织样本的简单且可靠的模型。
Effects of collagen digestion have been defined up to the fibril level. However, the question remains as to whether the alteration of skeletal muscle extracellular matrix (ECM) affects a muscle’s passive elastic response. Various elastography methods have been applied as tools for evaluating the mechanical properties and ECM content of skeletal muscle. In an effort to develop an ECM altered skeletal muscle model, this study determined the effect of collagen digestion on the passive elastic properties of skeletal muscle. Passive mechanical properties of rat diaphragms were evaluated in various degrees of collagen digestion. Between cyclic loading tests, muscle strips were immersed in various concentrations of clostridium histolyticum derived bacterial collagenase. All samples were later viewed via light microscopy. Cyclic testing revealed linear relationships between passive muscle stiffness and digestion time at multiple concentrations. These results demonstrate that collagenase digestion of the ECM in skeletal muscle could be used as a simple and reliable model of mechanically altered in vitro tissue samples.
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影响因子：
21.3
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通讯作者：
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