Alleviating Mechanical Allodynia and Modulating Cellular Immunity Contribute to Electroacupuncture's Dual Effect on Bone Cancer Pain.
Alleviating Mechanical Allodynia and Modulating Cellular Immunity Contribute to Electroacupuncture's Dual Effect on Bone Cancer Pain.
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减轻机械性异常性疼痛和调节细胞免疫有助于电针对骨癌疼痛的双重作用
DOI：
10.1177/1534735417728335
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发表时间：
2018-06
影响因子：
2.9
通讯作者：
Fang JQ
中科院分区：
文献类型：
--
作者：
Liang Y;Du JY;Fang JF;Fang RY;Zhou J;Shao XM;Jiang YL;Chen YT;Fang JQ
假设：电针（EA）已被用作替代镇痛治疗数百年，但其镇痛效力和治疗优势对骨癌疼痛（BCP）相比，吗啡仍不清楚。本研究旨在观察电针对BCP大鼠机械性痛觉超敏及细胞免疫功能的影响，并进一步探讨其可能的作用机制。方法：将步行者256乳腺癌细胞植入成年雌性SD大鼠左侧胫骨，建立BCP模型。电针组采用振荡波，频率2/100 Hz，强度0.5mA ~ 1 mA ~ 1.5mA，每次10 min，电针双侧足三里、昆仑穴30 min。电针刺激和吗啡（10 mg/kg，腹腔注射）隔日一次。电针前30分钟腹腔注射纳洛酮（0.3mg/kg）。用动态足底感觉仪测定大鼠的缩足阈值（PWTs），观察机械性痛觉超敏反应。分别采用WST-8法、流式细胞术和酶联免疫吸附法检测T细胞增殖、脾脏中CD 3+、CD 4+和CD 8 + T淋巴细胞百分比以及血浆中白细胞介素-2（IL-2）的表达。结果：胫骨内接种步行者256乳腺癌细胞显著降低了机械刺激的PWT。电针刺激可减轻BCP大鼠的机械性痛觉超敏反应，且电针的镇痛作用弱于吗啡。与吗啡相比，电针刺激BCP大鼠可增加ConA诱导的脾T细胞增殖和血浆IL-2含量，并增加脾CD 3 + CD 4+和CD 3 + CD 8 + T细胞亚群的百分比。腹腔注射纳洛酮可抑制电针的镇痛作用和部分免疫调节作用。结论：电针可明显减轻BCP所致的机械性痛觉超敏。电针的镇痛作用虽弱于吗啡，但对细胞免疫有调节作用。电针的镇痛和免疫调节作用可能通过阿片类物质介导的机制实现，有待进一步研究。
Hypothesis: Electroacupuncture (EA) has been used as an alternative analgesic therapy for hundreds of years, yet its analgesic potency and therapeutic advantage against bone cancer pain (BCP) in comparison with morphine remains unclear. This study aimed to investigate the effects of EA on mechanical allodynia and cellular immunity of BCP rats, and to further explore the potential mechanism. Methods: The BCP model was established by implanting Walker 256 mammary gland carcinoma cells into the left tibia of adult female Sprague-Dawley rats. EA (dilatational wave, 2/100 Hz, 0.5 mA–1mA–1.5 mA for 10 minutes each intensity) was applied bilaterally to Zusanli (ST 36) and Kunlun (BL 60) for 30 minutes. Both EA stimulation and morphine (10 mg/kg, intraperitoneally) was given once every other day. Naloxone (0.3 mg/kg, intraperitoneally) was injected at 30 minutes prior to EA. Mechanical allodynia were demonstrated by paw withdrawal thresholds (PWTs) which measured by dynamic plantar aesthesiometer. T cell proliferation, percentage of CD3+, CD4+ and CD8+ T lymphocytes in spleen as well as expression of interleukin-2 (IL-2) in plasma were detected by WST-8, flow cytometry, and enzyme-linked immunosorbent assay technique, respectively. Results: An intratibial inoculation of Walker 256 mammary gland carcinoma cells significantly decreased PWTs to mechanical stimuli. EA stimulation alleviated mechanical allodynia in BCP rats, and the analgesic potency of EA was weaker than that of morphine. In contrast to morphine, EA stimulation of BCP rats increased splenic concanavalin A (Con A)-induced T cell proliferation and plasma IL-2 content, as well as increased the percentages of splenic CD3+CD4+ and CD3+CD8+ T cell subsets. Moreover, both the analgesic effect and the partial immunomodulation of EA were suppressed by an intraperitoneal injection of naloxone. Conclusion: EA could significantly alleviate BCP-induced mechanical allodynia. Although the analgesic effect of EA was weaker than that of morphine, EA had an immunomodulation effect on cellular immunity. Both analgesic and immunomodulatory effect of EA might share the same mechanism via the opioid-mediated pathway, which needs further investigation.
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影响因子：
5.7
作者：
Cabioglu, Mehmet T.;Cetin, B. Eren
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
Ninković J;Roy S
通讯作者：
Roy S