De Novo Assembly of the Transcriptome for Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae) and Analysis on Insecticide Resistance-Related Genes.
De Novo Assembly of the Transcriptome for Oriental Armyworm Mythimna separata (Lepidoptera: Noctuidae) and Analysis on Insecticide Resistance-Related Genes.
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东方粘虫Mythimna separata（鳞翅目：夜蛾科）转录组的从头组装及杀虫剂抗性相关基因分析
DOI：
10.1093/jisesa/iew079
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Wuriyanghan H
中科院分区：
文献类型：
--
作者：
Liu Y;Qi M;Chi Y;Wuriyanghan H
粘虫步行者（Mythimna separata walker）是鳞翅目夜蛾科夜蛾属的一种多食性害虫，分布于近100科300多种粮食和经济作物。迄今为止，M. separata，严格限制了这种昆虫物种的分子生物学研究。在本研究中，我们对M.分离。测序及生物信息学分析共得到69,238个unigenes，其中45,227个unigenes通过blocking与数据库中的高同源基因进行了相应功能的注释，注释率为65.32%。几种鳞翅目昆虫与转录组数据最匹配。为深入了解粘虫抗药性的产生机制。分离，15个家族的基因编码的杀虫剂抗性相关蛋白进行了调查。在转录组数据中鉴定了这些家族中大量的unigenes，并且成功扩增了21个选定的unigenes中的17个。大多数这些基因的表达在幼虫阶段和肠道组织中检测到，这与它们在杀虫剂抗性中的假定参与是一致的。我们的研究为M.为夜蛾科其他昆虫提供了参考序列信息。
Mythimna separata walker (Lepidoptera: Noctuidae) is a polyphagous pest of nearly 100 families of more than 300 kinds of food and industrial crops. So far, both nucleotide and protein sequence information has been rarely available in database for M. separata, strictly limiting molecular biology research in this insect species. In this study, we carried out a transcriptome sequencing for M. separata. The sequencing and subsequent bioinformatics analysis yielded 69,238 unigenes, among which 45,227 unigenes were annotated to corresponding functions by blasting with high homologous genes in database, giving annotation rate of 65.32%. Several lepidopteran insects gave best matches with the transcriptome data. To gain insight into the mechanism of insecticide resistance in M. separata, 15 families of genes encoding insecticide resistance-related proteins were investigated. Substantial numbers of unigenes in these families were identified in the transcriptome data, and 17 out of 21 selected unigenes were successfully amplified. Expressions of most of these genes were detected at larval stages and in gut tissue, as was consistent with their putative involvement in insecticide resistance. Our study provides most comprehensive transcriptome data for M. separata to date, and also provides reference sequence information for other Noctuidae family insects.
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