Internalization of Sucrose by Methanococcus thermolithotrophicus
Internalization of Sucrose by Methanococcus thermolithotrophicus
复制标题
热石营养甲烷球菌对蔗糖的内化
DOI：
10.1128/aem.61.2.421-429.1995
复制
发表时间：
1995
影响因子：
4.4
通讯作者：
M. Roberts
中科院分区：
文献类型：
--
作者：
R. Ciulla;S. Krishnan;M. Roberts
当蔗糖存在于外部培养基中时，它被热嗜养甲烷球菌内化。蔗糖内化，由天然丰度（sup13）C核磁共振波谱和[(sup14)C]蔗糖摄取确定，与外部蔗糖水平成正比。吸收与能量无关，表现出与简单被动扩散过程一致的动力学行为。在蔗糖浓度为0.2 M时，随着外源培养基中NaCl浓度的增加，产甲烷受到抑制。当外部NaCl浓度为1.4 M时，0.2 M的蔗糖抑制了蛋白质含量的生长，这些结果很重要，因为它们表明：(i)蔗糖不能作为非带电溶质代替NaCl，在实验中评估外部渗透强度如何影响热嗜养m.s thermolithotrophicus的内部溶质组成；（ii）蔗糖不能作为细胞外体积的不渗透标记物，在实验中用于测量嗜热嗜养m.s hilothotrophicus的细胞内体积。
When sucrose is present in the external medium, it is internalized by Methanococcus thermolithotrophicus. Sucrose internalization, as determined by both natural abundance (sup13)C nuclear magnetic resonance spectroscopy and [(sup14)C]sucrose uptake, is directly proportional to external sucrose levels. The uptake is energy independent and exhibits kinetic behavior consistent with a simple passive diffusion process. In the presence of 0.2 M sucrose, methanogenesis is inhibited as the NaCl concentration in the external medium is increased. Growth, as determined by protein content, is inhibited by 0.2 M sucrose when the external NaCl concentration is 1.4 M. These results are important because they show that (i) sucrose cannot be used as a noncharged solute to replace NaCl in experiments to evaluate how external osmotic strength affects the internal solute composition of M. thermolithotrophicus, and (ii) sucrose cannot be used as an impermeable marker for the extracellular volume in experiments to measure the intracellular volume of M. thermolithotrophicus.
DOI：
10.1016/0167-4889(88)90138-3
发表时间：
1988-10
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
N. Belay;R. Sparling;B. Choi;M. Roberts;J. Roberts;L. Daniels
通讯作者：
N. Belay;R. Sparling;B. Choi;M. Roberts;J. Roberts;L. Daniels
产甲烷古细菌中的有机渗透剂。
DOI：
--
发表时间：
1991
期刊：
BioFactors (Oxford, England)
影响因子：
--
作者：
Robertson,DE;Roberts,MF
通讯作者：
Roberts,MF