Isolation of Marine Bacteria by In Situ Culture on Media-Supplemented Polyurethane Foam
Isolation of Marine Bacteria by In Situ Culture on Media-Supplemented Polyurethane Foam
复制标题
通过在补充培养基的聚氨酯泡沫上原位培养分离海洋细菌
作者：
Mina Yasumoto;M. Nishijima;M. K. Ngirchechol;K. Kanoh;Y. Shizuri;W. Miki
补充有各种琼脂培养基的聚氨酯泡沫（PUF）被用于原位捕获海洋细菌，因此它提供了一种基质，当它们暴露在珊瑚礁地区的天然海水中时，可以在其上进行培养。通过聚合酶链反应（PCR）扩增的16S rDNA片段的变性梯度凝胶电泳（DGGE）分析PUF块上的细菌种群。改变PUF块中培养基的组成和选择不同的采样位点导致在PUF块上检测到不同的细菌。例如，从PUF块中分离出利用铁（IU）的细菌、产生铁载体（SP）的细菌和石油降解（PD）的细菌，并且发现IU和SP含有铁，PD含有烃。该方法为分离新的有用的海洋细菌开辟了可能性。
Polyurethane foam (PUF) supplemented with various agar media was used in situ to trap marine bacteria and it consequently provided a substrate on which they could be cultivated while exposed to natural seawater in the coral reef area. The bacterial population on the PUF blocks was analyzed by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 16S rDNA fragments. Changing the composition of the cultivation medium in the PUF blocks and selecting different sampling sites resulted in different bacteria being detected on the PUF blocks. For example, iron-utilizing (IU) bacteria, siderophore-producing (SP) bacteria, and petroleum-degrading (PD) bacteria were isolated from PUF blocks and it was discovered that IU and SP contained iron and PD contained hydrocarbon. This method opens up the possibility for isolating novel and useful marine bacteria.
影响因子：
2.9
作者：
SCHWYN, B;NEILANDS, JB
通讯作者：
NEILANDS, JB