Manure contaminated with the antibiotic sulfadiazine impairs the abundance of nirK- and nirS-type denitrifiers in the gut of the earthworm Eisenia fetida

Manure contaminated with the antibiotic sulfadiazine impairs the abundance of nirK- and nirS-type denitrifiers in the gut of the earthworm Eisenia fetida
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被抗生素磺胺嘧啶污染的粪便会损害蚯蚓 Eisenia fetida 肠道中 nirK 和 nirS 型反硝化菌的丰度

DOI:
10.1007/s00374-009-0434-3
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发表时间:
2010
影响因子:
6.5
通讯作者:
Schloter
Schloter
中科院分区:
农林科学1区
文献类型:
--
作者:
Kotzerke;Kleineidam;Schloter

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磺胺嘧啶(SDZ)抗生素能影响土壤中的微生物。然而,迄今为止尚未描述对蚯蚓肠道中的发酵剂的影响。因此,SDZ污染的粪肥施加到土壤中的蚯蚓肠道中的硝化细菌的影响进行了评估,通过定量聚合酶链反应靶向基因编码的nirK-和nirS-型亚硝酸盐还原酶的硝化细菌。在仅施用有机肥的土壤中,蚯蚓消化道中nirK和nirS基因拷贝数分别为2.5 × 106和5.1 × 105。从粪便处理与SDZ的nirKandnirSin肠道内容物的拷贝数高达10倍less. Overall,数据表明SDZ对蚯蚓肠道中的消化酶的负面影响。
The antibiotic sulfadiazine (SDZ) can affect denitrifying bacteria in soil. However, effects on denitrifiers in the gut of earthworms have not been described so far. Therefore, the influence of SDZ-contaminated manure applied to soil on denitrifiers in the gut of the earthwormEisenia fetidawas assessed by quantitative polymerase chain reaction targeting genes coding fornirK- andnirS-type nitrite reductases of denitrifiers. Gut contents ofEisenia fetidacontained 2.5 × 106and 5.1 × 105gene copies ofnirKandnirS, respectively, after 2 weeks in soils amended with manure only. Copy numbers ofnirKandnirSin gut contents from manure treatments with SDZ were up to ten times less. Overall, the data indicate a negative impact of SDZ on denitrifiers in the gut of earthworms.
DOI: --
发表时间: 2006
影响因子: 4.4
作者:
M. Horn;R. Mertel;M. Gehre;M. Kästner;H. Drake
通讯作者: H. Drake
DOI: 10.1128/aem.00304-09
发表时间: 2009
影响因子: 4.4
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