Sequential Development of Hodgkin's Disease and CD30+ Diffuse Large B-Cell Lymphoma in a Patient With MALT-Type Lymphoma: Evidence of Different Clonal Origin of Single Microdissected Reed-Sternberg Cells
Sequential Development of Hodgkin's Disease and CD30+ Diffuse Large B-Cell Lymphoma in a Patient With MALT-Type Lymphoma: Evidence of Different Clonal Origin of Single Microdissected Reed-Sternberg Cells
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MALT 型淋巴瘤患者霍奇金病和 CD30 弥漫性大 B 细胞淋巴瘤的相继发展：单个显微解剖 Reed-Sternberg 细胞不同克隆起源的证据
DOI：
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发表时间：
2002
影响因子：
5.6
通讯作者：
J. Merlio
中科院分区：
文献类型：
--
作者：
M. Parrens;B. Vergier;O. Fitoussi;C. Lahet;G. Belléannée;G. Marit;P. Dubus;A. de Mascarel;M. Delfau;J. Merlio
我们在同一患者中观察到1994年出现扁桃体粘膜相关淋巴组织型淋巴瘤，1998年出现纵膈霍奇金病，2000年出现结肠CD 30+间变性弥漫性大B细胞淋巴瘤。一个相同大小的FR 3-JH片段被证明通过聚合酶链反应，无论是在总DNA和单个显微操作的细胞的水平，显示单核细胞样，里德-斯滕伯格，或间变性形态。此外，在粘膜相关淋巴组织型淋巴瘤和结肠CD 30+间变性弥漫性大B细胞淋巴瘤中检测到相同的IgH核苷酸序列，而纵隔霍奇金病IgH重排涉及不同的VH和JH基因。CD 30 + Reed-Sternberg和弥漫性大B细胞淋巴瘤细胞含有EB病毒EBER序列，在粘膜相关淋巴组织型淋巴瘤水平上未观察到。因此，EB病毒感染可能在弥漫性大B细胞淋巴瘤转化为粘膜相关淋巴组织型淋巴瘤和霍奇金病的淋巴瘤发生中起作用。除了它们不同的克隆来源，霍奇金病的Reed-Sternberg细胞表达CD 15+、CD 20+（罕见细胞）、CD 30+、Oct-2−、EBNA 2 −、LMP 1+表型，而弥漫性大B细胞淋巴瘤的间变性和Reed-Sternberg样细胞表达CD 15 −、CD 20+、CD 30+、Oct-2+、EBNA 2+和LMP 1+。有趣的是，我们还在最初的扁桃体粘膜相关淋巴组织型淋巴瘤中检测到分散的CD 30 + EB病毒-大细胞，这些细胞具有突出的核仁，这表明这些细胞可能易于EB病毒感染和/或大细胞转化。
We observed in the same patient the development of a tonsil mucosa-associated lymphoid tissue-type lymphoma in 1994, a mediastinal Hodgkin's disease in 1998, and a colonic CD30+ anaplastic diffuse large B-cell lymphoma in 2000. A same-sized FR3-JH fragment was demonstrated by polymerase chain reaction, both at the level of total DNA and of single micromanipulated cells, showing monocytoid, Reed-Sternberg, or anaplastic morphology. Moreover, an identical IgH nucleotide sequence was detected in mucosa-associated lymphoid tissue-type lymphoma and colonic CD30+ anaplastic diffuse large B-cell lymphoma, whereas mediastinal Hodgkin's disease IgH rearrangement involved different VH and JH genes. CD30+ Reed-Sternberg and diffuse large B-cell lymphoma cells contained Epstein-Barr virus EBER sequences that were not observed at the level of mucosa-associated lymphoid tissue-type lymphoma. Therefore, Epstein-Barr virus infection may have played a role in diffuse large B-cell lymphoma transformation of mucosa-associated lymphoid tissue-type lymphoma and in the lymphomagenesis of Hodgkin's disease. In addition to their different clonal origin, Reed-Sternberg cells of Hodgkin's disease expressed a CD15+, CD20+ (rare cells), CD30+, Oct-2−, EBNA2−, LMP1+ phenotype, whereas anaplastic and Reed-Sternberg-like cells of diffuse large B-cell lymphoma were CD15−, CD20+, CD30+, Oct-2+, EBNA2+, and LMP1+. Interestingly, we also detected scattered CD30+ Epstein-Barr virus− large cells with prominent nucleoli in the initial tonsil mucosa-associated lymphoid tissue-type lymphoma, suggesting that these cells could be prone to Epstein-Barr virus infection and/or large cell transformation.
DOI：
10.1073/pnas.89.13.5847
发表时间：
1992-07-01
影响因子：
11.1
作者：
ZHANG, L;CUI, XF;ARNHEIM, N
通讯作者：
ARNHEIM, N
所有具有聚合酶链式反应可放大 bcl-2 断点的晚期非霍奇金淋巴瘤在评估时和治疗后均具有含有 bcl-2 重排的残留细胞。
影响因子：
20.3
作者：
Gribben,JG;Freedman,As;Woo,SD;Blake,K;Shu,RS;Freeman,G;Longtine,JA;Pinkus,GS;Nadler,LM
通讯作者：
Nadler,LM