The genetic architecture of branched-chain amino acid accumulation in tomato fruits.
The genetic architecture of branched-chain amino acid accumulation in tomato fruits.
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DOI：
10.1093/jxb/err091
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发表时间：
2011-07
影响因子：
6.9
通讯作者：
Fernie AR
中科院分区：
文献类型：
--
作者：
Kochevenko A;Fernie AR
以往的研究水果代谢组成的遗传结构，使我们能够确定四个高度保守的支链氨基酸（BCAAs）的协调数量性状位点（QTL）。这项研究已经扩展到包括其他23种酶描述参与支链氨基酸的合成和降解途径。对这些酶的染色体位置进行粗略作图，可以确定24个基因的图谱位置。在这些基因中，8个共定位或定位接近BCAA QTL，包括编码酮醇酸还原异构酶（KARI）、二羟酸脱氢酶（DHAD）和异丙基苹果酸脱氢酶（IPMD）的那些。对这些基因表达水平的定量评估显示，S. IPMD的pennellii等位基因表现出该基因表达水平的变化，而KARI和DHAD的基因型之间的变化是不变的。而IPMD的反义抑制导致BCAA增加，KARI或DHAD的反义抑制都没有产生明显的效果，在水果BCAA含量。讨论了这些特定的酶在植物氨基酸代谢中的作用，并在目前的背景下了解植物支链氨基酸代谢的调节的结果。
Previous studies of the genetic architecture of fruit metabolic composition have allowed us to identify four strongly conserved co-ordinate quantitative trait loci (QTL) for the branched-chain amino acids (BCAAs). This study has been extended here to encompass the other 23 enzymes described to be involved in the pathways of BCAA synthesis and degradation. On coarse mapping the chromosomal location of these enzymes, it was possible to define the map position of 24 genes. Of these genes eight co-localized, or mapped close to BCAA QTL including those encoding ketol-acid reductoisomerase (KARI), dihydroxy-acid dehydratase (DHAD), and isopropylmalate dehydratase (IPMD). Quantitative evaluation of the expression levels of these genes revealed that the S. pennellii allele of IPMD demonstrated changes in the expression level of this gene, whereas those of KARI and DHAD were invariant across the genotypes. Whilst the antisense inhibition of IPMD resulted in increased BCAA, the antisense inhibition of neither KARI nor DHAD produced a clear effect in fruit BCAA contents. The results are discussed both with respect to the roles of these specific enzymes within plant amino acid metabolism and within the context of current understanding of the regulation of plant branched-chain amino acid metabolism.
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影响因子：
7.4
作者：
de Kraker, Jan-Willem;Luck, Katrin;Gershenzon, Jonathan
通讯作者：
Gershenzon, Jonathan
DOI：
10.1111/j.1432-1033.1990.tb15415.x
发表时间：
1990-03-10
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KANDRA, L;SEVERSON, R;WAGNER, GJ
通讯作者：
WAGNER, GJ
影响因子：
7.4
作者：
Do, Phuc Thi;Prudent, Marion;Fernie, Alisdair R.
通讯作者：
Fernie, Alisdair R.
影响因子：
4
作者：
Beck, HC;Hansen, AM;Lauritsen, FR
通讯作者：
Lauritsen, FR
影响因子：
4.3
作者：
Hagelstein, P;Sieve, B;Schultz, G
通讯作者：
Schultz, G