Nature-derived lignan compound VB-1 exerts hair growth-promoting effects by augmenting Wnt/β-catenin signaling in human dermal papilla cells.
Nature-derived lignan compound VB-1 exerts hair growth-promoting effects by augmenting Wnt/β-catenin signaling in human dermal papilla cells.
复制标题
天然木脂素化合物 VB-1 通过增强人真皮乳头细胞中的 Wnt/β-catenin 信号传导发挥促进毛发生长的作用
作者：
Luo J;Chen M;Liu Y;Xie H;Yuan J;Zhou Y;Ding J;Deng Z;Li J
牡荆素是一种木脂素类化合物，已被证明具有多种药理作用，例如抗炎、抗氧化和抗癌活性。然而，牡荆素对毛发再生的作用尚未阐明。
在用牡荆素化合物1（VB - 1）处理后，通过细胞计数和连续细胞培养检测人真皮乳头细胞（hDPCs）的增殖情况。通过逆转录聚合酶链反应（RT - PCR）检测lef1、wnt5a、bmp2、bmp4、alpl和vcan的表达。通过RT - PCR或免疫印迹法检测dkk1、tgf - β1、活性β - 连环蛋白和AXIN2的表达。在有或没有VB - 1的情况下测量毛干生长。
我们证明了在一定浓度范围内，VB - 1以浓度依赖的方式显著促进hDPCs的增殖。在所研究的毛发生长相关基因中，dkk1在经VB - 1处理的hDPCs中明显下调。活性β - 连环蛋白增加和AXIN2蛋白水平降低表明VB - 1在体外促进hDPCs中的Wnt/β - 连环蛋白信号传导。在VB - 1处理后，真皮乳头（DP）特征基因的表达也上调。我们的研究进一步通过毛囊器官培养实验表明VB - 1促进人毛囊（HF）生长。
VB - 1可能通过增强hDPCs中的Wnt/β - 连环蛋白信号传导来发挥促进毛发生长的作用。
Vitexin is a kind of lignan compound which has been shown to possess a variety of pharmacological effects, such as anti-inflammatory, anti-oxidative and anti-cancer activities. However the effect of vitexin on hair regeneration has not been elaborated. The proliferation of human dermal papilla cells (hDPCs) was examined by cell counting and continuous cell culture after vitexin compound 1 (VB-1) was treated. The expression of lef1, wnt5a, bmp2, bmp4, alpl and vcan was examined by RT-PCR. The expression of dkk1, tgf-β1, active-β-Catenin, and AXIN2 was examined by RT-PCR or immunoblotting. Hair shaft growth was measured in the absence or presence of VB-1. We demonstrated that VB-1 significantly promotes the proliferation of hDPCs in a concentration-dependent manner within a certain concentration range. Among the hair growth-related genes investigated, dkk1 was clearly down-regulated in hDPCs treated with VB-1. The increased active β-Catenin and decreased AXIN2 protein levels suggest that VB-1 facilitates Wnt/β-catenin signaling in hDPCs in vitro. The expression of DP signature genes was also upregulated after VB-1 treatment. Our study further indicated that VB-1 promotes human hair follicle (HF) growth by HF organ culture assay. VB-1 may exert hair growth-promoting effects via augmenting Wnt/β-catenin signaling in hDPCs.
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影响因子：
6.5
作者：
Li, Ji;Jiang, Ting Xin;Chuong, Cheng Ming
通讯作者：
Chuong, Cheng Ming
影响因子：
21.3
作者：
通讯作者：
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影响因子：
6.5
作者：
Kwack, Mi H.;Kim, Moon K.;Sung, Young K.
通讯作者：
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mTOR 信号传导通过平衡毛发再生过程中 BMP 介导的抑制来促进干细胞活化
影响因子：
5.5
作者：
Deng, Zhili;Lei, Xiaohua;Duan, Enkui
通讯作者：
Duan, Enkui
DOI：
10.1073/pnas.1309970110
发表时间：
2013-12-03
影响因子：
11.1
作者：
Higgins, Claire A.;Chen, James C.;Christiano, Angela M.
通讯作者：
Christiano, Angela M.