Combining Field-Enabled Capillary Vibrating Sharp-Edge Spray Ionization with Microflow Liquid Chromatography and Mass Spectrometry to Enhance 'Omics Analyses.
Combining Field-Enabled Capillary Vibrating Sharp-Edge Spray Ionization with Microflow Liquid Chromatography and Mass Spectrometry to Enhance 'Omics Analyses.
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DOI：
10.1021/jasms.0c00376
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发表时间：
2021-02-03
影响因子：
3.2
通讯作者：
Valentine SJ
中科院分区：
文献类型：
--
作者：
Majuta SN;DeBastiani A;Li P;Valentine SJ
现场启用毛细管振动锐边喷雾电离（cVSSI）与高流量液相色谱（LC）和质谱（MS）相结合，建立了当前的电离能力，用于代谢组学和蛋白质组学研究。比较了使用cVSSI和加热电喷雾电离（HESI）探针的实验，代表了最先进的微流LC-MS方法用于组学研究。对于代谢组学标准，cVSSI显示为负离子和正离子模式分析提供4±2的电离增强因子。对于胰凝乳肽，cVSSI在负离子模式和正离子模式分析中分别提供5±2和2±1的电离增强因子。在cVSSI数据集中观察到略宽的HPLC峰，一些研究表明这是由于分裂后流速略有下降。这可能是由于随着时间的推移，拉尖发射极部分受阻造成的。这种挑战可以通过使用在10到100 μL·min - 1流量范围内工作的LC泵来解决。在这个早期阶段，原理验证研究已经表明离子信号优于最先进的ESI，适用于各种正离子和负离子模式的分析物。总的来说，这代表了通过无电压cVSSI进行LC-MS分析的第一次演示的20到50倍的改进。对相同溶剂条件下洗脱的化合物的离子丰度进行单独比较，揭示了cVSSI和ESI之间电离效率的差异，并可能表明化合物不同的物理化学性质对电离的贡献不同。简要介绍了使用cVSSI在负离子和正离子模式分析中进一步增加电离增益的参数的未来研究。
Field-enabled capillary Vibrating Sharp-edge Spray Ionization (cVSSI) has been combined with high-flow liquid chromatography (LC) and mass spectrometry (MS) to establish current ionization capabilities for metabolomics and proteomics investigations. Comparisons are made between experiments employing cVSSI and a heated-electrospray ionization (HESI) probe representing the state-of-the-art in microflow LC-MS methods for ‘omics studies. For metabolomics standards, cVSSI is shown to provide an ionization enhancement by factors of 4±2 for both negative and positive ion mode analyses. For chymotryptic peptides, cVSSI is shown to provide an ionization enhancement by factors of 5±2 and 2±1 for negative and positive ion mode analyses, respectively. Slightly broader HPLC peaks are observed in the cVSSI datasets and several studies suggest that this results from a slightly decreased post-split flow rate. This may result from partial obstruction of the pulled-tip emitter over time. Such a challenge can be remedied with the usage of LC pumps that operate in the 10 to 100 μL·min−1 flow regime. At this early stage, the proof-of-principle studies already show ion signal advantages over state-of-the-art ESI for a wide variety of analytes in both positive and negative ion mode. Overall, this represents an ~20 to 50-fold improvement over the first demonstration of LC-MS analyses by voltage-free cVSSI. Separate comparisons of the ion abundances of compounds eluting under identical solvent conditions reveal ionization efficiency differences between cVSSI and ESI and may suggest varied contributions to ionization from different physicochemical properties of the compounds. Future investigations of parameters that could further increase ionization gains in negative and positive ion mode analyses with the use of cVSSI are briefly presented.
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