Proteomic analysis of microbial induced redox-dependent intestinal signaling.
Proteomic analysis of microbial induced redox-dependent intestinal signaling.
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DOI：
10.1016/j.redox.2018.11.011
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发表时间：
2019-01
期刊：
影响因子：
11.4
通讯作者：
Neish AS
中科院分区：
文献类型：
--
作者：
Matthews JD;Reedy AR;Wu H;Hinrichs BH;Darby TM;Addis C;Robinson BS;Go YM;Jones DP;Jones RM;Neish AS
肠道动态平衡在一定程度上是由与某些乳酸菌接触后在结肠粘膜产生的活性氧物种(ROS)调节的。从机制上讲，ROS可以通过氧化蛋白质中的半胱氨酸残基来调节蛋白质的功能。利用同位素编码亲和标记(ICATs)技术标记半胱氨酸的最新进展有助于识别半胱氨酸硫醇对刺激的修饰。在这里，我们使用ICATs绘制了结肠粘膜与鼠李糖乳杆菌GG(LGG)初次接触时氧化还原蛋白网络的图谱。我们在450多种蛋白质中检测到了显著的LGG特异性氧化还原变化，其中许多蛋白质参与了细胞过程，如内体运输、上皮细胞连接、屏障完整性以及细胞骨架的维持和形成。我们特别注意到了rac1的LGG特异性氧化，它是许多细胞过程的多效性调节因子。综上所述，这些数据揭示了对乳酸菌诱导的和氧化还原依赖的网络参与肠道内稳态的新见解。
Intestinal homeostasis is regulated in-part by reactive oxygen species (ROS) that are generated in the colonic mucosa following contact with certain lactobacilli. Mechanistically, ROS can modulate protein function through the oxidation of cysteine residues within proteins. Recent advances in cysteine labeling by the Isotope Coded Affinity Tags (ICATs) technique has facilitated the identification of cysteine thiol modifications in response to stimuli. Here, we used ICATs to map the redox protein network oxidized upon initial contact of the colonic mucosa with Lactobacillus rhamnosus GG (LGG). We detected significant LGG-specific redox changes in over 450 proteins, many of which are implicated to function in cellular processes such as endosomal trafficking, epithelial cell junctions, barrier integrity, and cytoskeleton maintenance and formation. We particularly noted the LGG-specific oxidation of Rac1, which is a pleiotropic regulator of many cellular processes. Together, these data reveal new insights into lactobacilli-induced and redox-dependent networks involved in intestinal homeostasis.
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DOI：
10.1016/j.alcohol.2008.12.009
发表时间：
2009-03
期刊：
Alcohol (Fayetteville, N.Y.)
影响因子：
--
作者：
Forsyth CB;Farhadi A;Jakate SM;Tang Y;Shaikh M;Keshavarzian A
通讯作者：
Keshavarzian A
影响因子：
7
作者：
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通讯作者：
Jones, Dean P.
影响因子：
8.8
作者：
Jones RM;Desai C;Darby TM;Luo L;Wolfarth AA;Scharer CD;Ardita CS;Reedy AR;Keebaugh ES;Neish AS
通讯作者：
Neish AS
影响因子：
4.4
作者：
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通讯作者：
Nusrat, Asma
影响因子：
8
作者：
通讯作者：
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