Ribosylhopane, a novel bacterial hopanoid, as precursor of C35 bacteriohopanepolyols in Streptomyces coelicolor A3(2).
Ribosylhopane, a novel bacterial hopanoid, as precursor of C35 bacteriohopanepolyols in Streptomyces coelicolor A3(2).
复制标题
DOI：
10.1002/cbic.201402261
复制
发表时间：
2014-09-22
期刊：
影响因子：
3.2
通讯作者：
Rohmer, Michel
中科院分区：
文献类型：
--
作者：
Liu, Wenjun;Sakr, Elias;Schaeffer, Philippe;Talbot, Helen M.;Donisi, Janina;Haertner, Thomas;Kannenberg, Elmar;Takano, Eriko;Rohmer, Michel
冷色链霉菌A3(2)野生型产生氨菌藿烷三醇作为唯一的细长的C35类藿烷。两个突变体的hopanoid表型携带先前鉴定的hopanoid生物合成基因簇的基因缺失，为C35细菌藿烷多元醇的形成提供了线索。Orf14编码一种假定的核苷酶：它的缺失导致不能转化为核糖体的腺苷藿烷的积累。Orf18编码一种推测的转氨酶：它的缺失导致腺苷藿烷、核糖素藿烷和细菌藿烷醇的积累。核糖体藿烷在20年前被假设为细菌类藿烷的前体，但从未在细菌中发现。orf18编码的转氨酶的缺失阻止了核糖藿烷还原胺化成氨细菌藿烷三醇并诱导其积累。它被醛糖还原酶类似的酶还原产生细菌藿烷醇，这通常不存在于葡萄球菌中。
Streptomyces coelicolor A3(2) wild type produces aminobacteriohopanetriol as the only elongated C35 hopanoid. The hopanoid phenotype of two mutants bearing a deletion of genes from a previously identified hopanoid biosynthesis gene cluster provides clues on the formation of C35 bacteriohopanepolyols. orf14 encodes a putative nucleosidase: its deletion induces the accumulation of adenosylhopane that cannot be converted into ribosylhopane. orf18 encodes a putative transaminase: its deletion results in the accumulation of adenosylhopane, ribosylhopane and bacteriohopanetetrol. Ribosylhopane was hypothesized twenty years ago as a precursor for bacterial hopanoids but was never identified in a bacterium. Absence of the transaminase encoded by orf18 prevents the reductive amination of ribosylhopane into aminobacteriohopanetriol and induces its accumulation. Its reduction by an aldose reductase like enzyme produces bacteriohopanetetrol, which is normally not present in S. coelicolor.
登录
查看更多内容
影响因子：
2.1
作者：
Poralla, K;Muth, G;Härtner, T
通讯作者：
Härtner, T
影响因子：
4.8
作者：
Hong, HJ;Hutchings, MI;Buttner, MJ
通讯作者：
Buttner, MJ
DOI：
10.1073/pnas.0337542100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Gust, B;Challis, GL;Chater, KF
通讯作者：
Chater, KF
影响因子：
15
作者：
BUDZIKIEWICZ, H;WILSON, JM;DJERASSI, C
通讯作者：
DJERASSI, C
影响因子：
3.6
作者：
BISSERET, P;ROHMER, M
通讯作者：
ROHMER, M