Identification of miRNAs involved in pear fruit development and quality.
Identification of miRNAs involved in pear fruit development and quality.
复制标题
梨果实发育和品质相关miRNA的鉴定
DOI：
10.1186/1471-2164-15-953
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发表时间：
2014-11-03
期刊：
影响因子：
4.4
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Wu J;Wang D;Liu Y;Wang L;Qiao X;Zhang S
研究背景MicroRNAs（miRNAs）是一类内源性小分子RNA，在植物中通过转录后水平介导基因表达而参与基因调控。先前的研究已经报道了从模式植物到多年生果树的各种植物中的miRNA鉴定。然而，miRNAs在梨果实发育中的作用尚不清楚。本文利用Illumina HiSeq 2000平台和生物信息学分析方法，对梨果实发育过程中不同时期的miRNA谱进行了研究。实时荧光定量PCR验证miRNAs的表达水平。结果梨中存在保守的和种特异性的miRNAs。从为开花后果实发育的不同阶段构建的六个小RNA文库中获得总读数，范围从19，030，925到25，576，773。比较分析表明，平均90个miRNAs的表达与不同的发育阶段之间的显着差异。通过对188个已知miRNAs的2,216个靶基因和184个新miRNAs的1,127个靶基因进行KEGG通路分析，发现miRNAs广泛参与果实发育的调控。其中11个miRNAs参与了木质素的合成，9个miRNAs参与了糖酸代谢，MiR160参与了生长素反应因子的调控。结论通过对梨果实发育过程中miRNAomes的比较分析，证明miRNAs广泛参与了果实发育和果实品质形成的调控。例如通过木质素合成、糖和酸代谢以及激素信号传导。结合计算分析和实验验证，为microRNA在梨等果实发育中的功能研究提供了有价值的信息。
BackgroundMicroRNAs (miRNAs) are a class of small, endogenous RNAs that take part in regulating genes through mediating gene expressions at the post-transcriptional level in plants. Previous studies have reported miRNA identification in various plants ranging from model plants to perennial fruit trees. However, the role of miRNAs in pear (Pyrus bretschneideri) fruit development is not clear. Here, we investigated the miRNA profiles of pear fruits from different time stages during development with Illumina HiSeq 2000 platform and bioinformatics analysis. Quantitative real-time PCR was used to validate the expression levels of miRNAs.ResultsBoth conserved and species-specific miRNAs in pear have been identified in this study. Total reads, ranging from 19,030,925 to 25,576,773, were obtained from six small RNA libraries constructed for different stages of fruit development after flowering. Comparative profiling showed that an average of 90 miRNAs was expressed with significant differences between various developmental stages. KEGG pathway analysis on 2,216 target genes of 188 known miRNAs and 1,127 target genes of 184 novel miRNAs showed that miRNAs are widely involved in the regulation of fruit development. Among these, a total of eleven miRNAs putatively participate in the pathway of lignin biosynthesis, nine miRNAs were identified to take part in sugar and acid metabolism, and MiR160 was identified to regulate auxin response factor.ConclusionComparative analysis of miRNAomes during pear fruit development is presented, and miRNAs were proved to be widely involved in the regulation of fruit development and formation of fruit quality, for example through lignin synthesis, sugar and acid metabolism, and hormone signaling. Combined with computational analysis and experimental confirmation, the research contributes valuable information for further functional research of microRNA in fruit development for pear and other species.
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影响因子：
56.9
作者：
Lagos-Quintana, M;Rauhut, R;Tuschl, T
通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
Johnson, Cameron;Bowman, Lewis;Adai, Alex T;Vance, Vicki;Sundaresan, Venkatesan
通讯作者：
Sundaresan, Venkatesan
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
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