Deformation Microscopy for Dynamic Intracellular and Intranuclear Mapping of Mechanics with High Spatiotemporal Resolution.
Deformation Microscopy for Dynamic Intracellular and Intranuclear Mapping of Mechanics with High Spatiotemporal Resolution.
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DOI：
10.1016/j.celrep.2019.04.009
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发表时间：
2019-04-30
期刊：
影响因子：
8.8
通讯作者：
Neu CP
中科院分区：
文献类型：
--
作者：
Ghosh S;Seelbinder B;Henderson JT;Watts RD;Scott AK;Veress AI;Neu CP
Structural heterogeneity is a hallmark of living cells that drives local mechanical properties and dynamic cellular responses. However, the robust quantification of intracellular mechanics is lacking from conventional methods. Here, we describe the development of deformation microscopy, which leverages conventional imaging and an automated hyperelastic warping algorithm to investigate strain history, deformation dynamics, and changes in structural heterogeneity within the interior of cells and cell nuclei. Using deformation microscopy, we found that partial or complete disruption of LINC complexes in cardiomyocytes in vitro and lamin A/C deficiency in myocytes in vivo abrogate dominant tensile loading in the nuclear interior. We also found that cells cultured on stiff substrates or in hyperosmotic conditions displayed abnormal strain burden and asymmetries at interchromatin regions, which are associated with active transcription. Deformation microscopy represents a foundational approach toward intracellular elastography, with the potential utility to provide mechanistic and quantitative insights in diverse mechanobiological applications. Ghosh et al. show that deformation microscopy, a technique based on image analysis and mechanics, reveals deformation dynamics and structural heterogeneity changes for several applications and at multiple scales, including tissues, cells, and nuclei. They reveal how the disruption of nuclear proteins and pathological conditions abrogate mechanical strain in the nuclear interior.
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DOI：
10.1186/1755-1536-5-15
发表时间：
2012-09-03
期刊：
Fibrogenesis & tissue repair
影响因子：
--
作者：
Fan D;Takawale A;Lee J;Kassiri Z
通讯作者：
Kassiri Z
影响因子：
41.2
作者：
Ihalainen TO;Aires L;Herzog FA;Schwartlander R;Moeller J;Vogel V
通讯作者：
Vogel V
影响因子：
9.2
作者：
Isermann, Philipp;Lammerding, Jan
通讯作者：
Lammerding, Jan
影响因子：
112.7
作者：
Iskratsch, Thomas;Wolfenson, Haguy;Sheetz, Michael P.
通讯作者：
Sheetz, Michael P.
影响因子：
41.2
作者：
Dingal PC;Bradshaw AM;Cho S;Raab M;Buxboim A;Swift J;Discher DE
通讯作者：
Discher DE