Effect of scatter factor on motility of epithelial cells and fibroblasts
Effect of scatter factor on motility of epithelial cells and fibroblasts
复制标题
散射因子对上皮细胞和成纤维细胞运动的影响
DOI：
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发表时间：
1989
影响因子：
5.6
通讯作者：
M. Stoker
中科院分区：
文献类型：
--
作者：
M. Stoker
分散因子是由成纤维细胞释放的蛋白质，其引起上皮细胞集落的分散和细胞间连接的破坏，以及伪足的起皱和快速延伸和移动的形态改变。为了确定分散因子是否对细胞迁移有直接影响，使用Boyden小室试验来确定部分纯化的因子对细胞通过8 μm孔径过滤器的迁移的影响。结果表明，Madin达比犬肾（MDCK）细胞的迁移率受到刺激，通常在100 ng/ml时达到最大值，根据细胞的形态和排列，这相当于标准试验中的100至200单位活性。迁移是由于趋化性和趋化作用。角质形成细胞系也是敏感的，在较小程度上，BSCI猴肾细胞。然而，新鲜分离的成纤维细胞和成纤维细胞系的运动性没有受到显著影响。结果证实了散射因子的细胞特异性和旁分泌作用，并表明这种成纤维细胞衍生分子可以直接刺激上皮细胞的运动。
The scatter factor is a protein released by fibroblasts that causes dispersal of epithelial cell colonies and disruption of intercellular junctions, as well as an alteration of morphology with ruffling and rapid extension and movement of pseudopodia. To find out if the scatter factor has a direct effect on cell migration, the Boyden chamber assay was used to determine the effect of partially purified factor on the migration of cells through 8 μm pore size filters. The results showed that the mobility of Madin‐Darby canine kidney (MDCK) cells was stimulated, and usually maximal at 100 ng per ml, which is equivalent to 100 to 200 units of activity in the standard assay based on the morphology and arrangement of cells. The migration was due to chemotaxis and chemokinesis. A keratinocyte cell line was also sensitive as were, to a lesser extent, BSCI monkey kidney cells. The motility of freshly isolated fibroblasts and fibroblast cell lines, however, was not significantly affected. The results confirm the cell specificity and paracrine role of the scatter factor and show that this fibroblast‐derived molecule can directly stimulate the movement of epithelial cells.
大鼠乳腺癌细胞克隆对肿瘤源性细胞因子的趋化反应。
DOI：
10.1016/0006-291x(87)90746-7
发表时间：
1987
影响因子：
3.1
作者：
Atnip,KD;Carter,LM;Nicolson,GL;Dabbous,MK
通讯作者：
Dabbous,MK