Genome-wide transcriptional response of Trichoderma reesei to lignocellulose using RNA sequencing and comparison with Aspergillus niger.
Genome-wide transcriptional response of Trichoderma reesei to lignocellulose using RNA sequencing and comparison with Aspergillus niger.
复制标题
利用 RNA 测序以及与黑曲霉的比较研究毛霉菌对木质纤维素的全基因组转录反应。
DOI：
10.1186/1471-2164-14-541
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发表时间：
2013-08-09
期刊：
影响因子：
4.4
通讯作者：
Archer DB
中科院分区：
文献类型：
--
作者：
Ries L;Pullan ST;Delmas S;Malla S;Blythe MJ;Archer DB
第二代生物燃料生产的主要部分是将木质纤维素生物质酶糖化成可发酵糖。许多真菌产生的酶可以将几种真菌的木质纤维素和鸡尾酒糖化，包括经过充分研究的物种，如里氏木霉和黑曲霉，可以在商业上用于这一过程。当面对复杂的木质纤维素材料时，这种商业上可用的酶鸡尾酒不一定代表真菌本身使用的一系列酶。研究人员利用RNA-seq技术探索了稻霉暴露于麦秸后基因的全局诱导，并与已发表的RNA-seq数据和稻霉感知和响应麦秸的模型进行了比较。在T. reesei中，编码已知和预测的细胞壁降解酶的转录物水平在暴露于秸秆24小时后非常高（约占总mRNA的13%），但低于在A. niger中记录的水平（约占总mRNA的19%）。进一步分析发现，在这两种生物中，来自相同糖苷水解酶家族但不同碳水化合物酯酶和多糖裂解酶家族的酶均上调。在黑曲霉中也发现了可能在促进黑曲霉碳水化合物分解中起作用的辅助蛋白，其诱导的酶种类与黑曲霉相似。与黑曲霉相似，T. reesei中也存在反义转录物，其表达受生长条件的调节。T. reesei使用类似于a . niger的一系列酶来分解固体木质纤维素底物。这表明在这两种腐殖真菌中对木质纤维素的降解有一种保守的策略。该研究为进一步分析和表征在木质纤维素底物存在下高度诱导的基因提供了基础。这些数据将有助于阐明T. reesei对固体基质的识别和随后的降解机制，并为有效生产第二代生物燃料提供有用的信息。
A major part of second generation biofuel production is the enzymatic saccharification of lignocellulosic biomass into fermentable sugars. Many fungi produce enzymes that can saccarify lignocellulose and cocktails from several fungi, including well-studied species such as Trichoderma reesei and Aspergillus niger, are available commercially for this process. Such commercially-available enzyme cocktails are not necessarily representative of the array of enzymes used by the fungi themselves when faced with a complex lignocellulosic material. The global induction of genes in response to exposure of T. reesei to wheat straw was explored using RNA-seq and compared to published RNA-seq data and model of how A. niger senses and responds to wheat straw. In T. reesei, levels of transcript that encode known and predicted cell-wall degrading enzymes were very high after 24 h exposure to straw (approximately 13% of the total mRNA) but were less than recorded in A. niger (approximately 19% of the total mRNA). Closer analysis revealed that enzymes from the same glycoside hydrolase families but different carbohydrate esterase and polysaccharide lyase families were up-regulated in both organisms. Accessory proteins which have been hypothesised to possibly have a role in enhancing carbohydrate deconstruction in A. niger were also uncovered in T. reesei and categories of enzymes induced were in general similar to those in A. niger. Similarly to A. niger, antisense transcripts are present in T. reesei and their expression is regulated by the growth condition. T. reesei uses a similar array of enzymes, for the deconstruction of a solid lignocellulosic substrate, to A. niger. This suggests a conserved strategy towards lignocellulose degradation in both saprobic fungi. This study provides a basis for further analysis and characterisation of genes shown to be highly induced in the presence of a lignocellulosic substrate. The data will help to elucidate the mechanism of solid substrate recognition and subsequent degradation by T. reesei and provide information which could prove useful for efficient production of second generation biofuels.
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影响因子：
6.3
作者：
Kubicek CP;Mikus M;Schuster A;Schmoll M;Seiboth B
通讯作者：
Seiboth B
DOI：
10.1111/j.1757-1707.2009.01004.x
发表时间：
2009-02-18
期刊：
Global change biology. Bioenergy
影响因子：
--
作者：
Dodd D;Cann IK
通讯作者：
Cann IK
细胞胞胞成虫酶糖基化对生物量转化的影响。
影响因子：
6.3
作者：
Jeoh, Tina;Michener, William;Himmel, Michael E.;Decker, Stephen R.;Adney, William S.
通讯作者：
Adney, William S.
DOI：
10.1046/j.1432-1033.2002.03199.x
发表时间：
2002-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
de Vries, RP;Parenicová, L;Visser, J
通讯作者：
Visser, J
影响因子：
5
作者：
Ike, Masakazu;Park, Jeung-yil;Tokuyasu, Ken
通讯作者：
Tokuyasu, Ken