Reperfusion and the plasma isoforms of creatine kinase isoenzymes: a clinical perspective.
Reperfusion and the plasma isoforms of creatine kinase isoenzymes: a clinical perspective.
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再灌注和肌酸激酶同工酶的血浆亚型：临床视角。
DOI：
10.1016/s0735-1097(87)80406-0
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发表时间：
1987
影响因子：
24
通讯作者：
R. Roberts
中科院分区：
文献类型：
--
作者：
R. Roberts
三种 CK 同工酶。肌酸激酶（CK）同工酶的引入和应用显着提高了心肌梗死（I）检测的敏感性和特异性。血浆肌酸激酶 MB 分数 (MB CK) 升高目前被认为是诊断急性心肌梗死最敏感、最特异且最具成本效益的方法 (2, 3)。最近血浆 CK 同工酶亚型的纯化、表征和检测为进一步改进和新应用提供了希望。肌酸激酶由两个亚基组成，每个亚基的分子量约为 41,000。 M 亚基因其在肌肉中的丰富性而被命名为 MM CK，它是心肌和骨骼肌中的主要同工酶。 B 亚基在大脑中最丰富，形成 BB CK，其少量也存在于胃肠道等器官中。混合形式的MB CK，除了痕量外，主要存在于心脏中，其中它约占总心肌CK活性的15%，其余为MM CK。因此，MB CK 的特异性与心肌损伤相关。两个亚基 M 和 B 结合形成三个异酶。在常规条件下的电泳过程中，MM 在原点基本上保持中性，而带负电的 MB 和 BB 则表现出阳极迁移。在 20 世纪 60 年代和 1970 年代，偶尔有报道出现额外的同工酶，但这些通常被当作人工制品丢弃。 1977 年，Wevers 等人(4) 表明，MM CK 在释放到血液中后，在电泳上显示出三个条带。他们提出了令人信服的证据，证明血液以某种方式诱导了两个 MM CK 分子的形成，其阳极迁移量比从组织中释放的形式更多。
The three CK isoenzymes. The introduction and application of creatine kinase (CK) isoenzymes significantly improved the sensitivity and specificity of detection of myocardial infarction (I). Elevated plasma creatine kinase, MB fraction (MB CK) is now regarded as the most sensitive, specific and cost-effective means of diagnosing acute myocardial infarction (2, 3). The recent purification, characterization and detection of plasma CK isoenzyme subforms offers promise for further improvement and new applications. Creatine kinase is composed of two subunits, each with a molecular weight of about 41,000. The subunit M, so designated because of its abundance in muscle, forms MM CK, the predominant isoenzyme in cardiac and skeletal muscle. The B subunit, most abundant in the brain, forms BB CK, which in minimal amounts is also present in organs such as the gastrointestinal tract. The hybrid form MB CK is, except for trace amounts, found primarily in the heart, where it comprises about 15% of total myocardial CK activity, the remainder being MM CK. Hence, the specificity of MB CK is relative for myocardial injury. The two subunits M and B combine to form three isoenzyrnes. During electrophoresis under conventional conditions, MM remains essentially neutral at the origin, whereas negatively charged MB and BB exhibit anodal migration. In the 1960s and 1970s occasional reports appeared of additional isoenzymes but these were usually discarded as artifact. In 1977, Wevers et al.(4) showed that MM CK, on release into the blood, exhibited three bands on electrophoresis. They presented convincing evidence that blood somehow induced the formation of two MM CK molecules with more anodal migration than the form released from the tissue.
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狗心脏肌酸激酶 mRNA 的完整核苷酸序列：物种内和物种间氨基酸序列的保守性。
DOI：
10.1073/pnas.82.24.8394
发表时间：
1985
影响因子：
11.1
作者：
Roman,D;Billadello,J;Gordon,J;Grace,A;Sobel,B;Strauss,A
通讯作者：
Strauss,A
影响因子：
37.8
作者：
Allan S. Jaffe;Harvey Serota;Ann M. Grace;B. Sobel
通讯作者：
Allan S. Jaffe;Harvey Serota;Ann M. Grace;B. Sobel
肌酸激酶蛋白序列由加州鱼雷电器官 mRNA 制成的 cDNA 编码。
DOI：
10.1073/pnas.81.22.7007
发表时间：
1984
影响因子：
11.1
作者：
West,BL;Babbitt,PC;Mendez,B;Baxter,JD
通讯作者：
Baxter,JD
影响因子：
37.8
作者：
HACKEL, DB;REIMER, KA;ROBERTS, R
通讯作者：
ROBERTS, R
天然存在和体外诱导的多种形式 MM 肌酸激酶的纯化和表征。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
George,S;Ishikawa,Y;Perryman,MB;Roberts,R
通讯作者：
Roberts,R