Three amino acid residues bind corn odorants to McinOBP1 in the polyembryonic endoparasitoid of Macrocentrus cingulum Brischke.
Three amino acid residues bind corn odorants to McinOBP1 in the polyembryonic endoparasitoid of Macrocentrus cingulum Brischke.
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DOI：
10.1371/journal.pone.0093501
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bai SX
中科院分区：
文献类型：
--
作者：
Ahmed T;Zhang TT;Wang ZY;He KL;Bai SX
气味结合蛋白（OBP）在将气味分子从感器淋巴液转运到嗅觉受体以启动行为反应中起核心作用。本研究利用大肠杆菌表达并经镍离子亲和层析纯化了带茧蜂OBP。实时PCR实验表明，McinOBP 1主要在成虫触角中表达，表达水平因性别而异。以N-苯基-萘胺（1-NPN）为荧光探针进行的配体结合实验表明，McinOBP 1不仅可以与绿叶挥发物（包括醛类和萜类）结合，而且还可以与脂肪醇结合，其亲和力顺序为反式-2-壬烯醛>顺式-3-己烯-1-醇>反式-丁香酚，表明McinOBP 1在一般气味化学感受中的作用。我们选择了这三种气味剂进行进一步的同源性建模和基于其结合亲和力的配体对接。Val 58、Leu 62和Glu 130是与这三种气味剂结合的结合口袋中的关键氨基酸。三个突变体，Val 58，Leu 62和Glu 130，其中缬氨酸，亮氨酸和谷氨酸残基分别被丙氨酸，脯氨酸和丙氨酸取代，显示出降低的亲和力，这些气味。这一信息表明，Val 58，Leu 62和Glu 130参与这些化合物的结合，可能是通过与配体官能团形成氢键的配体的特异性识别。
Odorant binding proteins (OBPs) play a central role in transporting odorant molecules from the sensillum lymph to olfactory receptors to initiate behavioral responses. In this study, the OBP of Macrocentrus cingulum McinOBP1 was expressed in Escherichia coli and purified by Ni ion affinity chromatography. Real-time PCR experiments indicate that the McinOBP1 is expressed mainly in adult antennae, with expression levels differing by sex. Ligand-binding experiments using N-phenyl-naphthylamine (1-NPN) as a fluorescent probe demonstrated that the McinOBP1 can bind green-leaf volatiles, including aldehydes and terpenoids, but also can bind aliphatic alcohols with good affinity, in the order trans-2-nonenal>cis-3-hexen-1-ol>trans-caryophelle, suggesting a role of McinOBP1 in general odorant chemoreception. We chose those three odorants for further homology modeling and ligand docking based on their binding affinity. The Val58, Leu62 and Glu130 are the key amino acids in the binding pockets that bind with these three odorants. The three mutants, Val58, Leu62 and Glu130, where the valine, leucine and glutamic residues were replaced by alanine, proline and alanine, respectively; showed reduced affinity to these odorants. This information suggests, Val58, Leu62 and Glu130 are involved in the binding of these compounds, possibly through the specific recognition of ligands that forms hydrogen bonds with the ligands functional groups.
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影响因子：
4.4
作者：
Andersson MN;Grosse-Wilde E;Keeling CI;Bengtsson JM;Yuen MM;Li M;Hillbur Y;Bohlmann J;Hansson BS;Schlyter F
通讯作者：
Schlyter F
DOI：
10.1016/s0006-291x(02)00185-7
发表时间：
2002-04-26
影响因子：
3.1
作者：
Ban, LP;Zhang, L;Pelosi, P
通讯作者：
Pelosi, P
DOI：
10.1073/pnas.0501447102
发表时间：
2005-04-12
影响因子：
11.1
作者：
Leal, WS;Chen, AM;Tsuruda, JM
通讯作者：
Tsuruda, JM
影响因子：
2.2
作者：
Edwards, OR;Hopper, KR
通讯作者：
Hopper, KR
影响因子：
5.6
作者：
Damberger, Fred F.;Ishida, Yuko;Wuethrich, Kurt
通讯作者：
Wuethrich, Kurt