Antennal transcriptome analysis of the chemosensory gene families in the tree killing bark beetles, Ips typographus and Dendroctonus ponderosae (Coleoptera: Curculionidae: Scolytinae).
Antennal transcriptome analysis of the chemosensory gene families in the tree killing bark beetles, Ips typographus and Dendroctonus ponderosae (Coleoptera: Curculionidae: Scolytinae).
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DOI：
10.1186/1471-2164-14-198
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发表时间：
2013-03-21
期刊：
影响因子：
4.4
通讯作者：
Schlyter F
中科院分区：
文献类型：
--
作者：
Andersson MN;Grosse-Wilde E;Keeling CI;Bengtsson JM;Yuen MM;Li M;Hillbur Y;Bohlmann J;Hansson BS;Schlyter F
欧洲云杉树皮甲虫 Ipstypographus 和北美山松甲虫 Dendroctonus ponderosae（鞘翅目：象甲科：小蜂亚科）是针叶林的严重害虫。这两种树皮甲虫都利用聚集信息素来协调对寄主树的大规模攻击，而来自寄主树和非寄主树的气味剂则调节信息素反应。因此，树皮甲虫的嗅觉对于健康至关重要。然而，有关树皮甲虫嗅觉检测基因的信息一直缺乏，并且在鞘翅目中也很有限。我们从 I.typographus 和 D.ponderosae 的下一代测序中组装了触角转录组，以鉴定主要化学感应多基因家族的成员。基因本体（GO）注释表明，两个物种中与特定 GO 术语相关的转录本的相对丰度高度相似。在这两个物种中都发现了与嗅觉功能相关的术语的转录本。重点关注化学感应基因家族，我们在 I.tyrographus 中鉴定了 15 个假定的气味结合蛋白 (OBP)、6 个化学感应蛋白 (CSP)、3 个感觉神经元膜蛋白 (SNMP)、43 个气味受体 (OR)、6 个味觉受体 (GR) 和 7 个离子型受体 (IR)；黄松中 31 个假定的 OBP、11 个 CSP、3 个 SNMP、49 个 OR、2 个 GR 和 15 个 IR。将预测的蛋白质序列与面粉甲虫、赤拟谷盗、天牛、Megacyllene caryae 和果蝇、果蝇中的对应蛋白质序列进行比较。最显着的结果是在 OR 中发现的，其中发现了大的树皮甲虫特异性扩展。然而，一些进化枝包含来自所有四种甲虫物种的受体，表明一些鞘翅目 OR 谱系之间存在一定程度的保守性。二氧化碳的推定 GR 和保守触角 IR 的直系同源物包含在已识别的受体组中。现已在三个鞘翅目物种（ORs 的四个物种）中鉴定出对化学感受重要的蛋白质家族。因此，这项研究可以改进鞘翅目嗅觉的进化分析。在两种最具破坏性的森林害虫中鉴定这些蛋白质（共享许多化学信息素）尤其重要，因为它们可能代表种群控制的新目标。
The European spruce bark beetle, Ips typographus, and the North American mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae: Scolytinae), are severe pests of coniferous forests. Both bark beetle species utilize aggregation pheromones to coordinate mass-attacks on host trees, while odorants from host and non-host trees modulate the pheromone response. Thus, the bark beetle olfactory sense is of utmost importance for fitness. However, information on the genes underlying olfactory detection has been lacking in bark beetles and is limited in Coleoptera. We assembled antennal transcriptomes from next-generation sequencing of I. typographus and D. ponderosae to identify members of the major chemosensory multi-gene families. Gene ontology (GO) annotation indicated that the relative abundance of transcripts associated with specific GO terms was highly similar in the two species. Transcripts with terms related to olfactory function were found in both species. Focusing on the chemosensory gene families, we identified 15 putative odorant binding proteins (OBP), 6 chemosensory proteins (CSP), 3 sensory neuron membrane proteins (SNMP), 43 odorant receptors (OR), 6 gustatory receptors (GR), and 7 ionotropic receptors (IR) in I. typographus; and 31 putative OBPs, 11 CSPs, 3 SNMPs, 49 ORs, 2 GRs, and 15 IRs in D. ponderosae. Predicted protein sequences were compared with counterparts in the flour beetle, Tribolium castaneum, the cerambycid beetle, Megacyllene caryae, and the fruit fly, Drosophila melanogaster. The most notable result was found among the ORs, for which large bark beetle-specific expansions were found. However, some clades contained receptors from all four beetle species, indicating a degree of conservation among some coleopteran OR lineages. Putative GRs for carbon dioxide and orthologues for the conserved antennal IRs were included in the identified receptor sets. The protein families important for chemoreception have now been identified in three coleopteran species (four species for the ORs). Thus, this study allows for improved evolutionary analyses of coleopteran olfaction. Identification of these proteins in two of the most destructive forest pests, sharing many semiochemicals, is especially important as they might represent novel targets for population control.
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影响因子：
9.8
作者：
Benton, Richard;Sachse, Silke;Michnick, Stephen W;Vosshall, Leslie B
通讯作者：
Vosshall, Leslie B
DOI：
10.1046/j.1432-1327.1999.00438.x
发表时间：
1999-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Angeli, S;Ceron, F;Pelosi, P
通讯作者：
Pelosi, P
影响因子：
2.6
作者：
Bohbot, J.;Pitts, R. J.;Zwiebel, L. J.
通讯作者：
Zwiebel, L. J.
影响因子：
3.8
作者：
Engsontia, Patamarerk;Sanderson, Alan P.;Brown, Stephen
通讯作者：
Brown, Stephen
影响因子：
--
作者：
Bogdanova, Ekaterina A.;Shagin, Dmitry A.;Lukyanov, Sergey A.
通讯作者：
Lukyanov, Sergey A.