Investigating spermatogenesis in Drosophila melanogaster.
Investigating spermatogenesis in Drosophila melanogaster.
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DOI:
10.1016/j.ymeth.2014.04.020
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发表时间:
2014-06-15
期刊:
影响因子:
4.8
通讯作者:
Jones, D. Leanne
中科院分区:
文献类型:
--
作者:
Demarco, Rafael S.;Eikenes, Asmund H.;Haglund, Kaisa;Jones, D. Leanne
The process of spermatogenesis in Drosophila melanogaster provides a powerful model system to probe a variety of developmental and cell biological questions, such as the characterization of mechanisms that regulate stem cell behavior, cytokinesis, meiosis, and mitochondrial dynamics. Classical genetic approaches, together with binary expression systems, FRT-mediated recombination, and novel imaging systems to capture single cell behavior, are rapidly expanding our knowledge of the molecular mechanisms regulating all aspects of spermatogenesis. This methods chapter provides a detailed description of the system, a review of key questions chapter that have been addressed or remain unanswered thus far, and an introduction to tools and techniques available to probe each stage of spermatogenesis.
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