Steps of nuclear pore complex disassembly and reassembly during mitosis in early Drosophila embryos.
Steps of nuclear pore complex disassembly and reassembly during mitosis in early Drosophila embryos.
复制标题
早期果蝇胚胎有丝分裂过程中核孔复合体的拆卸和重组步骤。
DOI：
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发表时间：
2001
影响因子：
4
通讯作者：
Martin W. Goldberg
中科院分区：
文献类型：
--
作者：
Elena Kiseleva;S. Rutherford;L. Cotter;Terence D. Allen;Martin W. Goldberg
核孔复合物（NPC）在体内有丝分裂过程中的组装和拆卸机制尚不清楚。为了解决这个问题，并确定NPC拆卸和组装的步骤，我们使用场发射扫描电子显微镜（FESEM）、高分辨率表面成像技术和透射电子显微镜研究了果蝇早期发育合胞阶段的胚胎核结构。合胞胚胎的核分裂以半闭合有丝分裂为特征，在此过程中，核膜仅在极区破裂，并排列成由额外的“纺锤体包膜”包围的内双层膜。从分裂核表面观察到的这一过程步骤的FESEM分析证实了我们之前通过一系列结构中间体组装npc的体外模型，首次显示了从一个中间体到下一个中间体的时间进展。新生的非核细胞最初出现在有丝分裂的核包膜和上面的纺锤体膜之间的融合点。提出了一种NPC分解模型，该模型从释放中心转运蛋白和去除星环之前的细胞质环亚基开始。
The mechanisms of nuclear pore complex (NPC) assembly and disassembly during mitosis in vivo are not well defined. To address this and to identify the steps of the NPC disassembly and assembly, we investigated Drosophila embryo nuclear structure at the syncytial stage of early development using field emission scanning electron microscopy (FESEM), a high resolution surface imaging technique, and transmission electron microscopy. Nuclear division in syncytial embryos is characterized by semi-closed mitosis, during which the nuclear membranes are ruptured only at the polar regions and are arranged into an inner double membrane surrounded by an additional 'spindle envelope'. FESEM analysis of the steps of this process as viewed on the surface of the dividing nucleus confirm our previous in vitro model for the assembly of the NPCs via a series of structural intermediates, showing for the first time a temporal progression from one intermediate to the next. Nascent NPCs initially appear to form at the site of fusion between the mitotic nuclear envelope and the overlying spindle membrane. A model for NPC disassembly is offered that starts with the release of the central transporter and the removal of the cytoplasmic ring subunits before the star ring.
影响因子：
3
作者：
North,SimonJ;Koles,Kate;Hembd,Caleb;Morris,HowardR;Dell,Anne;Panin,VladislavM;Haslam,StuartM
通讯作者：
Haslam,StuartM
盘基网柄菌双特异性激酶 splA 对于孢子分化至关重要。
DOI：
10.1242/dev.122.10.3295
发表时间：
1996
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Nuckolls,GH;Osherov,N;Loomis,WF;Spudich,JA
通讯作者：
Spudich,JA