Improved adductor function after canine recurrent laryngeal nerve injury and repair using muscle progenitor cells.
Improved adductor function after canine recurrent laryngeal nerve injury and repair using muscle progenitor cells.
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DOI：
10.1002/lary.26992
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Halum S
中科院分区：
文献类型：
--
作者：
Paniello RC;Brookes S;Bhatt NK;Bijangi-Vishehsaraei K;Zhang H;Halum S
肌肉祖细胞(MPC)可以从肌肉样本中分离出来，并在培养过程中生长到临界质量。当它们被移植到大鼠喉部肌肉中时，它们被证明能够存活和整合。在本研究中，在犬模型上测试了MPC植入物增强神经支配的甲状旁肌内收肌功能的能力。动物研究。取自3只犬的胸锁乳突肌标本。4~5周分离MPC，培养至107个细胞，切断修复同侧喉返神经后植入右侧甲状旁肌内。左侧经历了同样的神经损伤，但没有植入细胞。在治疗前和治疗6个月后再次测量喉内收肌的力量，并采集肌肉进行组织学检查。从所有的狗身上成功地培养出了MPC。未植入对照组的喉内收肌力量测量值平均为治疗前的60%，植入MPC后为98%，植入运动终板增强的MPC后为128%。组织学证实，植入的MPC存活下来，整合到甲状旁肌纤维中，并与神经末梢密切接触，表明具有功能性神经支配。在这项先导性的犬类研究中，MPC被证明显著增强内收肌功能。针对患者的MPC植入有可能用于改善声带麻痹/瘫痪、萎缩和其他疾病患者的喉功能。进一步的实验也在计划之中。N/A(动物研究)
Muscle progenitor cells (MPCs) can be isolated from muscle samples and grown to a critical mass in culture. They have been shown to survive and integrate when implanted into rat laryngeal muscles. In this study, the ability of MPC implants to enhance adductor function of reinnervated thyroarytenoid muscles was tested in a canine model. Animal study. Sternocleidomastoid muscle samples were harvested from three canines. MPCs were isolated and cultured to 107 cells over 4–5 weeks, then implanted into right thyroarytenoid muscles after ipsilateral recurrent laryngeal nerve transection and repair. The left sides underwent the same nerve injury, but no cells were implanted. Laryngeal adductor force was measured pre-treatment and again six months later, and the muscles were harvested for histology. MPCs were successfully cultured from all dogs. Laryngeal adductor force measurements averaged 60% of their baseline pre-treatment values in non-implanted controls, 98% after implantation with MPCs, and 128% after implantation with motor endplate-enhanced MPCs. Histology confirmed the implanted MPCs survived, became integrated into thyroarytenoid muscle fibers, and were in close contact with nerve endings, suggesting functional innervation. MPCs were shown to significantly enhance adductor function in this pilot canine study. Patient-specific MPC implantation could potentially be used to improve laryngeal function in patients with vocal fold paresis/paralysis, atrophy, and other conditions. Further experiments are planned. N/A (animal study)
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影响因子：
2.6
作者：
Halum, Stacey L.;McRae, Bryan;Bijangi-Vishehsaraei, Khadijeh;Hiatt, Kelly
通讯作者：
Hiatt, Kelly
影响因子：
3.4
作者：
Dahm, JD;Paniello, RC
通讯作者：
Paniello, RC
影响因子：
2.6
作者：
Paniello, Randal C.;Rich, Jason T.;Debnath, Nick L.
通讯作者：
Debnath, Nick L.
影响因子：
2.6
作者：
Halum, Stacey L.;Naidu, Moumita;Hingtgen, Cynthia M.
通讯作者：
Hingtgen, Cynthia M.
影响因子：
2.6
作者：
Halum, Stacey L.;Hiatt, Kelly K.;Clapp, D. Wade
通讯作者：
Clapp, D. Wade