The putative single-stranded DNA-binding protein of the filamentous bacteriophage, If1. Amino acid sequence of the protein and structure of the gene

The putative single-stranded DNA-binding protein of the filamentous bacteriophage, If1. Amino acid sequence of the protein and structure of the gene
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丝状噬菌体的推定单链 DNA 结合蛋白 If1。

DOI:
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发表时间:
1991
期刊:
Proceedings of the Royal Society of London. Series B: Biological Sciences
影响因子:
--
通讯作者:
G. B. Petersen
G. B. Petersen
中科院分区:
--
文献类型:
--
作者:
A. Carne;D. Hill;P. Stockwell;G. Hughes;G. B. Petersen

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已从感染的细菌细胞中分离出位于噬菌体Ifl基因组区域的基因对应的蛋白产物,该基因含有迄今研究的所有丝状噬菌体单链DNA (ssDNA)结合蛋白的编码,并确定了其氨基酸序列。成熟蛋白含有95个氨基酸(计算分子质量为10553 Da)。其序列与DNA序列预测一致,但缺乏起始蛋氨酸残基。尽管噬菌体Ifl蛋白与其他已研究的丝状噬菌体的ssdna结合蛋白之间几乎没有直接的序列同源性,基于计算机的各种物理和结构参数比较表明,噬菌体Ifl蛋白含有一个与噬菌体T4基因32蛋白和假单胞菌噬菌体Pfl ssdna结合蛋白的结构域密切相关的结构域,并表明Ifl蛋白确实具有ssdna结合功能,尽管我们无法直接证明这一点。
The protein product corresponding to the gene located in the region of the coliphage Ifl genome shown to contain the code for the single-stranded DNA (ssDNA)-binding proteins of all filamentous phages so far studied has been isolated from infected bacterial cells and its amino acid sequence determined. The mature protein contains 95 amino acids (calculated molecular mass 10553 Da). Its sequence corresponds to that predicted from the DNA sequence but lacks the initiating methionine residue. Although there is little direct sequence homology between the phage Ifl protein and the ssDNA-binding proteins of the other filamentous phages that have been studied, computer-based comparisons of various physical and structural parameters showed that the phage Ifl protein contains a domain that is closely related to domains in the coliphage T4 gene 32 protein and the Pseudomonas phage Pfl ssDNA-binding protein and suggest that the Ifl protein does have a ssDNA-binding function although we were unable to show this directly.
丝状细菌病毒 If1 和 Ike 主要外壳蛋白的一级结构。
DOI: --
发表时间: 1981
期刊: The Journal of biological chemistry
影响因子: --
作者:
Nakashima,Y;Frangione,B;Wiseman,RL;Konigsberg,WH
通讯作者: Konigsberg,WH
噬菌体 f1 形态发生信号和基因 V 蛋白/噬菌体单链 DNA 复合物。
DOI: 10.1016/0042-6822(84)90399-4
发表时间: 1984
期刊: Virology
影响因子: 3.7
作者:
Grant,RA;Webster,RE
通讯作者: Webster,RE
DOI: 10.1016/0022-2836(87)90268-3
发表时间: 1987
影响因子: 5.6
作者:
Prasad,BV;Chiu,W
通讯作者: Chiu,W
丝状病毒 Pf3 的主要外壳蛋白和单链 DNA 结合蛋白。
DOI: 10.1073/pnas.81.3.699
发表时间: 1984
影响因子: 11.1
作者:
Putterman,DG;Casadevall,A;Boyle,PD;Yang,HL;Frangione,B;Day,LA
通讯作者: Day,LA