E-cigarette constituents propylene glycol and vegetable glycerin decrease glucose uptake and its metabolism in airway epithelial cells in vitro.
E-cigarette constituents propylene glycol and vegetable glycerin decrease glucose uptake and its metabolism in airway epithelial cells in vitro.
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DOI：
10.1152/ajplung.00123.2020
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发表时间：
2020-12-01
期刊：
影响因子：
--
通讯作者：
Baines DL
中科院分区：
文献类型：
--
作者：
Woodall M;Jacob J;Kalsi KK;Schroeder V;Davis E;Kenyon B;Khan I;Garnett JP;Tarran R;Baines DL
电子尼古丁输送系统，或电子香烟，利用通常含有丙二醇（PG）和植物甘油（VG）的液体溶液来产生蒸汽，并作为尼古丁和调味剂的载体。有证据表明，这些“载体”降低了包括气道上皮细胞在内的上皮细胞的生长和存活。我们假设3% PG或PG与VG混合（3% PG/VG, 55:45）抑制人类气道上皮细胞的葡萄糖摄取，这是降低气道细胞存活率的第一步。H441或人细支气管上皮细胞（HBECs）暴露于PG和PG/VG（30-60分钟）可抑制葡萄糖摄取和线粒体ATP合成。PG/VG抑制糖酵解。PG/VG和甘露醇降低了气液界面培养的细胞体积和高度。甘露醇增加p38 MAPK磷酸化，而PG/VG不增加。PG/VG降低了经上皮电阻，这与经上皮溶质渗透率增加有关。PG/VG降低了绿色荧光蛋白连接的葡萄糖转运体GLUT1和GLUT10光漂白后的荧光恢复，表明葡萄糖转运功能受到损害。将PG/VG蒸汽雾化到原代HBECs的顶端表面10分钟，模拟电子烟吸烟的效果，也减少了葡萄糖的运输。总之，短期暴露于电子烟的关键成分PG/VG，会降低气道细胞的葡萄糖运输和代谢。我们认为这是PG/VG减少细胞体积和膜流动性的结果，并进一步影响上皮屏障功能。综合这些结果，我们认为这些因素有助于降低上皮的防御特性。我们认为，反复/长期暴露于这些物质可能会导致电子烟使用者的气道损伤。
Electronic nicotine delivery systems, or e-cigarettes, utilize a liquid solution that normally contains propylene glycol (PG) and vegetable glycerin (VG) to generate vapor and act as a carrier for nicotine and flavorings. Evidence indicated these “carriers” reduced growth and survival of epithelial cells including those of the airway. We hypothesized that 3% PG or PG mixed with VG (3% PG/VG, 55:45) inhibited glucose uptake in human airway epithelial cells as a first step to reducing airway cell survival. Exposure of H441 or human bronchiolar epithelial cells (HBECs) to PG and PG/VG (30–60 min) inhibited glucose uptake and mitochondrial ATP synthesis. PG/VG inhibited glycolysis. PG/VG and mannitol reduced cell volume and height of air-liquid interface cultures. Mannitol, but not PG/VG, increased phosphorylation of p38 MAPK. PG/VG reduced transepithelial electrical resistance, which was associated with increased transepithelial solute permeability. PG/VG decreased fluorescence recovery after photobleaching of green fluorescent protein-linked glucose transporters GLUT1 and GLUT10, indicating that glucose transport function was compromised. Puffing PG/VG vapor onto the apical surface of primary HBECs for 10 min to mimic the effect of e-cigarette smoking also reduced glucose transport. In conclusion, short-term exposure to PG/VG, key components of e-cigarettes, decreased glucose transport and metabolism in airway cells. We propose that this was a result of PG/VG reduced cell volume and membrane fluidity, with further consequences on epithelial barrier function. Taking these results together, we suggest these factors contribute to reduced defensive properties of the epithelium. We propose that repeated/chronic exposure to these agents are likely to contribute to airway damage in e-cigarette users.
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影响因子：
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作者：
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DOI：
10.1016/0005-2736(91)90097-r
发表时间：
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期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
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