Correlation of interferon-induced expression of MxA mRNA in peripheral blood mononuclear cells with the response of patients with chronic active hepatitis C to IFN-alpha therapy.
Correlation of interferon-induced expression of MxA mRNA in peripheral blood mononuclear cells with the response of patients with chronic active hepatitis C to IFN-alpha therapy.
复制标题
干扰素诱导的外周血单核细胞中 MxA mRNA 表达与慢性活动性丙型肝炎患者对 IFN-α 治疗的反应的相关性。
DOI：
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复制
发表时间：
1999
影响因子：
2.3
通讯作者：
Ferdinando Dianzani
中科院分区：
文献类型：
--
作者：
Guido Antonelli;E. Simeoni;O. Turriziani;R. Tesoro;Alessandro Redaelli;L. Roffi;L. Antonelli;Mauro Pistello;Ferdinando Dianzani
MxA是一种对某些病毒具有选择性活性的蛋白质，是公认的I型干扰素（IFN）活性的特异性指标。我们开发了一种内部控制的定量竞争PCR来测量外周血单核细胞（PBMC）中MxA mRNA的表达量。这种分析是更敏感的，定量的，并且易于应用于系列临床样品比以前描述的方法。我们对27例给予干扰素α 2治疗的慢性活动性丙型肝炎患者进行了回顾性分析。大多数这样的患者没有获得持续的好处，但却遭受了治疗的副作用、费用和不便。在6个月的治疗期结束时，27人中有14人根据临床理由被分类为有反应者，13人被分类为无反应者。我们测量了在IFN-alpha2治疗前后8周获得的PBMC中的MxA mRNA。所有患者在治疗前均表达一定水平的mRNA，治疗8周后，19例患者mRNA水平升高。这种增加仅在被分类为应答者的患者中是显著的（p < 0.001）。这强烈表明，在ifn - α治疗期间，丙型肝炎病毒（HCV）患者PBMC中MxA mRNA表达量增加最有可能获得长期益处。如果这一发现在未来的前瞻性研究中得到证实，它将为HCV患者管理ifn - α治疗提供一个极其重要的预测指标。
MxA, a protein with selective activity against certain viruses, is an accepted specific indicator of type I interferon (IFN) activity. We have developed an internally controlled quantitative-competitive PCR to measure the amounts of MxA mRNA expressed in peripheral blood mononuclear cells (PBMC). This assay is more sensitive, quantitative, and easily applied to serial clinical samples than previously described methods. We have applied this assay retrospectively to 27 patients with chronic active hepatitis C given IFN-alpha2. Most such patients gain no sustained benefit but nevertheless suffer from the side effects, expense, and inconvenience of the treatment. Fourteen of the 27 had been classified on clinical grounds as responders and 13 as nonresponders at the end of a 6 month treatment period. We measured MxA mRNA in PBMC obtained before and after 8 weeks of IFN-alpha2 treatment. All the patients expressed some level of mRNA before treatment began, and after 8 weeks of treatment, the level rose in 19. This increase was significant (p < 0.001) only in patients classified as responders. This strongly suggests that hepatitis C virus (HCV) patients who express increased amounts of MxA mRNA in their PBMC during IFN-alpha treatment are most likely to obtain long-term benefit. If this finding is confirmed in future prospective studies, it will provide an extremely important predictive marker for managing IFN-alpha therapy in patients with HCV.
MxA 过度表达揭示了四个范可尼贫血互补组中常见的遗传联系。
DOI：
10.1172/jci119836
发表时间：
1997
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Li,Y;Youssoufian,H
通讯作者：
Youssoufian,H
干扰素和人 MxA 抑制人副流感病毒 3 复制。
DOI：
10.1006/viro.1996.0321
发表时间：
1996
期刊：
Virology.
影响因子：
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作者：
Zhao,H;De,BP;Das,T;Banerjee,AK
通讯作者：
Banerjee,AK