Antigenic evolution of H9N2 chicken influenza viruses isolated in China during 2009-2013 and selection of a candidate vaccine strain with broad cross-reactivity.
Antigenic evolution of H9N2 chicken influenza viruses isolated in China during 2009-2013 and selection of a candidate vaccine strain with broad cross-reactivity.
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DOI：
10.1016/j.vetmic.2015.10.031
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发表时间：
2016
影响因子：
3.3
通讯作者：
Pu, Juan
中科院分区：
文献类型：
--
作者：
Wei, Yandi;Xu, Guanlong;Zhang, Guozhong;Wen, Chu;Anwar, Furkat;Wang, Shuoguo;Lemmon, Gordon;Wang, Jinliang;Carter, Robert;Wang, Min;Sun, Honglei;Sun, Yipeng;Zhao, Jixun;Wu, Gang;Webster, Robert G.;Liu, Jinhua;Pu, Juan
我们此前曾证明，1994-2008年分离的H9N2亚型禽流感病毒(AIV)进化为不同的抗原组(C、D和E)，然后经历了商业疫苗的抗原性漂移，导致2010-2013年全国暴发。在这项研究中，用一组多克隆抗体进行血凝抑制和中和试验，对2009-2013年从鸡中分离的H9N2型禽流感病毒进行抗原性分析。我们的发现证实了最近的H9N2病毒从商业疫苗中分离出来的抗原性漂移，并表明这些抗原变异体大部分形成了一个新的HI抗原群F，少数属于D和E群，F群病毒的抗原性有轻微的变异。从血凝素(HA)基因序列推导出的氨基酸序列的遗传分析表明，2009-2013年流感病毒的15个突变中有9个突变可以定位到已知的抗原点，这可能是F群新的抗原性的原因。这些抗原性变化使得有必要修改流感疫苗以确保有效的保护。选择了候选疫苗ck/Heb/YT/10，在减少病毒脱落方面对来自不同抗原组的病毒提供了显著的保护，这表明存在广泛的交叉反应。综上所述，我们的结果表明，中国的H9N2型鸡流感病毒已从不同的抗原群进化为一个新的F群，该群在全国暴发期间成为主导，目前似乎正在经历新的抗原分化。系统监测和及时更新疫苗毒株对今后的病毒预防和控制非常重要。
We previously demonstrated that H9N2 subtype avian influenza viruses (AIVs) isolated from 1994 to 2008 evolved into distinct antigenic groups (C, D, and E) and then underwent antigenic drift from commercial vaccines, causing a country-wide outbreak during 2010–2013. In this study, H9N2 AIVs isolated from chickens during 2009–2013 were antigenically analyzed by performing hemagglutination inhibition and neutralization assays using a panel of polyclonal antibodies. Our findings confirmed the antigenic drift of recent H9N2 viruses from the commercial vaccine and showed that most of these antigenic variants form a novel HI antigenic group, F, with a few belonging to groups D and E. Slight antigenic variation was observed in group F viruses. Genetic analysis of amino acid sequences deduced from hemagglutinin (HA) gene sequences indicated that 9 of 15 mutations predominant in the 2009–2013 viruses can be mapped to known antigenic sites, which might be responsible for the novel antigenicity of group F. These antigenic changes make it necessary to modify the influenza vaccine to ensure efficient protection. A vaccine candidate, Ck/HeB/YT/10, was selected and provided significant protection against viruses from different antigenic groups in terms of reduction in virus shedding, suggesting broad cross-reactivity. Taken together, our results indicate that the H9N2 chicken influenza viruses in China have evolved from distinct antigenic groups into a novel group F that became dominant during the country-wide outbreak and now seems to be undergoing new antigenic divergence. Systematic surveillance and timely updating of vaccine strains are important for viral prevention and control in the future.
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影响因子：
4.3
作者：
Cai Z;Zhang T;Wan XF
通讯作者：
Wan XF
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影响因子：
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作者：
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作者：
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通讯作者：
Holmes, Edward C.
影响因子：
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作者：
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