Comparative phosphoproteomic analysis of blast resistant and susceptible rice cultivars in response to salicylic acid
Comparative phosphoproteomic analysis of blast resistant and susceptible rice cultivars in response to salicylic acid
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抗稻瘟病和感稻瘟病水稻品种对水杨酸响应的比较磷酸蛋白质组学分析
DOI：
10.1186/s12870-019-2075-5
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发表时间：
2019-10
影响因子：
5.3
通讯作者：
Nie Yanfang
中科院分区：
文献类型：
--
作者：
Sun Ranran;Qin Shiwen;Zhang Tong;Wang Zhenzhong;Li Huaping;Li Yunfeng;Nie Yanfang
研究背景水杨酸（SA）是诱导水稻抗病原菌入侵的重要信号分子。蛋白质磷酸化在植物防御反应中起着重要的调节作用，而水稻对SA介导的防御反应的整体磷酸化蛋白质组变化尚未见报道。在这项研究中，比较磷酸化蛋白质组分析进行了双向凝胶电泳（2-DE）和质谱（MS）分析，与两个近等基因水稻品种后SA treatment.Results37磷蛋白点差异表达SA处理后，其中29个被确定为MALDI-TOF/TOF MS，属于9个功能类别。两个品种中光合作用相关磷蛋白、抗氧化酶、分子伴侣的表达相似，表明SA可能缓解了植物光合作用的降低，调节了抗氧化防御活性，从而提高了两个品种的基础抗性反应。与此同时，与防御、糖代谢、蛋白质合成和降解相关的磷蛋白在两个品种中的差异表达，表明SA介导的磷酸化调节可能协调了两个品种复杂的细胞活动。此外，磷酸化位点的四个鉴定磷蛋白进行了验证NanoLC-MS/MS，和磷酸化调节的三种酶（肉桂酰辅酶A还原酶，磷酸甘油酸酯酶和抗坏血酸过氧化物酶）进行了验证活性determination.ConclusionsOur研究表明，磷酸化调节SA介导的可能有助于不同的抗性反应的两个品种。据我们所知，这是第一个报告，以测量水稻磷酸蛋白质组的变化，响应SA，这提供了新的见解SA诱导水稻防御的分子机制。
BackgroundSalicylic acid (SA) is a significant signaling molecule that induces rice resistance against pathogen invasion. Protein phosphorylation carries out an important regulatory function in plant defense responses, while the global phosphoproteome changes in rice response to SA-mediated defense response has not been reported. In this study, a comparative phosphoproteomic profiling was conducted by two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) analysis, with two near-isogenic rice cultivars after SA treatment.ResultsThirty-seven phosphoprotein spots were differentially expressed after SA treatment, twenty-nine of which were identified by MALDI-TOF/TOF MS, belonging to nine functional categories. Phosphoproteins involved in photosynthesis, antioxidative enzymes, molecular chaperones were similarly expressed in the two cultivars, suggesting SA might alleviate decreases in plant photosynthesis, regulate the antioxidant defense activities, thus improving basal resistance response in both cultivars. Meanwhile, phosphoproteins related to defense, carbohydrate metabolism, protein synthesis and degradation were differentially expressed, suggesting phosphorylation regulation mediated by SA may coordinate complex cellular activities in the two cultivars. Furthermore, the phosphorylation sites of four identified phosphoproteins were verified by NanoLC-MS/MS, and phosphorylated regulation of three enzymes (cinnamoyl-CoA reductase, phosphoglycerate mutase and ascorbate peroxidase) was validated by activity determination.ConclusionsOur study suggested that phosphorylation regulation mediated by SA may contribute to the different resistance response of the two cultivars. To our knowledge, this is the first report to measure rice phosphoproteomic changes in response to SA, which provides new insights into molecular mechanisms of SA-induced rice defense.
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影响因子：
5.3
作者：
通讯作者：
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影响因子：
3.7
作者：
Kim Y;Mun BG;Khan AL;Waqas M;Kim HH;Shahzad R;Imran M;Yun BW;Lee IJ
通讯作者：
Lee IJ
影响因子：
3.3
作者：
Dezhuo Pan;Lingxia Wang;Fanglin Tan;Si Lu;Xiaojie Lv;M. Zaynab;Chi-Lien Cheng;Y. Abubakar;
通讯作者：
Dezhuo Pan;Lingxia Wang;Fanglin Tan;Si Lu;Xiaojie Lv;M. Zaynab;Chi-Lien Cheng;Y. Abubakar;
影响因子：
3.4
作者：
Agrawal, GK;Thelen, JJ
通讯作者：
Thelen, JJ
影响因子：
56.9
作者：
CHEN, ZX;SILVA, H;KLESSIG, DF
通讯作者：
KLESSIG, DF