Neonatal hypoxia ischemia redistributes L1 cell adhesion molecule into rat cerebellar lipid rafts.
Neonatal hypoxia ischemia redistributes L1 cell adhesion molecule into rat cerebellar lipid rafts.
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DOI：
10.1038/s41390-022-01974-4
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发表时间：
2022-11
影响因子：
3.6
通讯作者：
Bearer, Cynthia F.
中科院分区：
文献类型：
--
作者：
Waddell, Jaylyn;Rickman, Nicholas C.;He, Min;Tang, Ningfeng;Bearer, Cynthia F.
缺氧缺血性脑病（Hypoxic-ischemic encephalopathy，HIE）是一种严重的致残性疾病。低温是目前唯一的治疗方法。足月时，新生儿小脑可能特别容易受到HIE的影响。此时，许多发育过程依赖于脂筏功能。质膜的这些微区对于细胞信号传导和轴突延伸至关重要。我们推测HIE改变了小脑中脂筏的蛋白质含量。出生后第10天（PN）的动物（被认为与人类相当）通过右颈动脉结扎后缺氧进行缺氧缺血性（HI）损伤。对于一些动物，在PN 7上施用LPS，并且在缺氧后进行低温（HT）4小时。从右侧和左侧小脑分离脂筏。在缺氧后4和72 h测定L1细胞粘附分子在脂筏中的百分比。HI单独引起L1在脂筏中的百分比显著增加，这种增加在右侧小脑中持续到72 h，但在左侧小脑中没有。HI后72小时，在左侧小脑中检测到LPS的小但显著的作用。低温没有影响。脂筏可能成为新生儿缺氧缺血性脑病干预的新靶点。
Hypoxic-ischemic encephalopathy (HIE) is a devastating disease with lifelong disabilities. Hypothermia is currently the only treatment. At term, the neonatal cerebellum may be particularly vulnerable to the effects of HIE. At this time, many developmental processes depend on lipid raft function. These microdomains of the plasma membrane are critical for cellular signaling and axon extension. We hypothesized that HIE alters the protein content of lipid rafts in the cerebellum. Postnatal day (PN) 10 animals, considered human term equivalent, underwent hypoxic-ischemic (HI) injury by a right carotid artery ligation followed by hypoxia. For some animals, LPS was administered on PN7 and hypothermia (HT) was conducted for 4 hours post-hypoxia. Lipid rafts were isolated from right and left cerebella. The percent of total L1 cell adhesion molecule in lipid rafts was determined 4 and 72 h after hypoxia. No sex differences were found. HI alone caused significant increases in the percent of L1 in lipid rafts which persisted until 72 h in the right but not the left cerebellum. A small but significant effect of LPS was detected in the left cerebellum 72 h after HI. Hypothermia had no effect. Lipid rafts may be a new target for interventions of HIE.
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影响因子：
6.1
作者：
Biran, Valerie;Heine, Vivi M.;Verney, Catherine;Sheldon, R. Ann;Spadafora, Ruggero;Vexler, Zinaida S.;Rowitch, David H.;Ferriero, Donna M.
通讯作者：
Ferriero, Donna M.
影响因子：
2.1
作者：
Fatemi A;Wilson MA;Johnston MV
通讯作者：
Johnston MV
影响因子：
3.4
作者：
Eklind, S;Mallard, C;Hagberg, H
通讯作者：
Hagberg, H
DOI：
10.1016/j.bbamem.2015.10.009
发表时间：
2016-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Hou TY;Barhoumi R;Fan YY;Rivera GM;Hannoush RN;McMurray DN;Chapkin RS
通讯作者：
Chapkin RS
影响因子：
2.5
作者：
Berman, Deborah R.;Mozurkewich, Ellen;Silverstein, Faye S.
通讯作者：
Silverstein, Faye S.