Fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation.
Fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation.
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DOI：
10.3389/fpls.2015.00089
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发表时间：
2015
影响因子：
5.6
通讯作者：
De Gara L
中科院分区：
文献类型：
--
作者：
Cimini S;Locato V;Vergauwen R;Paradiso A;Cecchini C;Vandenpoel L;Verspreet J;Courtin CM;D'Egidio MG;Van den Ende W;De Gara L
小麦籽粒含有果聚糖，即具有益生元特性的基于果糖的低聚糖，其含量取决于籽粒的发育阶段在2重量%和35重量%之间。为了提高对导致果聚糖贮藏和降解的代谢途径的认识，分析了硬粒小麦籽粒发育过程中发生的碳水化合物通量。在不同的发育阶段收集籽粒，并进行碳水化合物（单糖和二糖、果聚糖、淀粉）的定性定量分析，同时分析参与其生物合成和水解的酶的活性和基因表达。硬粒小麦籽粒中果聚糖的高分辨HPAEC-PAD分析表明，籽粒发育初期果聚糖含量较高，主要是二叉糖和1，1-nystose等聚合度较高的果聚糖，籽粒成熟过程中果聚糖库的变化可能是影响籽粒发育过程中碳水化合物代谢和贮藏的信号途径之一。在第一个发展阶段果聚糖积累可能有助于使内核更有效的Suc汇和参与渗透调节，而观察到的减少其内容可能标志着过渡到后期的发展阶段，过渡也是精心策划的氧化还原平衡的变化。
Wheat kernels contain fructans, fructose based oligosaccharides with prebiotic properties, in levels between 2 and 35 weight % depending on the developmental stage of the kernel. To improve knowledge on the metabolic pathways leading to fructan storage and degradation, carbohydrate fluxes occurring during durum wheat kernel development were analyzed. Kernels were collected at various developmental stages and quali-quantitative analysis of carbohydrates (mono- and di-saccharides, fructans, starch) was performed, alongside analysis of the activities and gene expression of the enzymes involved in their biosynthesis and hydrolysis. High resolution HPAEC-PAD of fructan contained in durum wheat kernels revealed that fructan content is higher at the beginning of kernel development, when fructans with higher DP, such as bifurcose and 1,1-nystose, were mainly found. The changes in fructan pool observed during kernel maturation might be part of the signaling pathways influencing carbohydrate metabolism and storage in wheat kernels during development. During the first developmental stages fructan accumulation may contribute to make kernels more effective Suc sinks and to participate in osmotic regulation while the observed decrease in their content may mark the transition to later developmental stages, transition that is also orchestrated by changes in redox balance.
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影响因子：
7.4
作者：
Kötting, O;Pusch, K;Ritte, G
通讯作者：
Ritte, G
影响因子：
4.3
作者：
De Roover, J;Vandenbranden, K;Van den Ende, W
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Van den Ende, W
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影响因子：
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De Gara, L.
影响因子：
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通讯作者：
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