Modulation of human gingival fibroblast cell metabolism by methyl mercaptan.
Modulation of human gingival fibroblast cell metabolism by methyl mercaptan.
复制标题
甲硫醇对人牙龈成纤维细胞代谢的调节。
DOI：
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发表时间：
1992
影响因子：
3.5
通讯作者：
J. Tonzetich
中科院分区：
文献类型：
--
作者：
P. Johnson;W. Ng;J. Tonzetich
甲硫醇(CH3SH)是一种恶臭化合物，在活动性牙周病患者的口腔和裂隙空气中含量升高。由于它可能在疾病过程中发挥作用，因此使用人牙龈成纤维细胞培养和活的猪未角化口腔粘膜组织切片来评估其效果。结果表明，经[14C]标记的甘氨酸和脯氨酸冲击的CH3SH暴露细胞的蛋白质含量降低了约25%。此外，这种有害影响在随后在对照95%空气/5%CO2无硫醇环境中孵育24小时的测试培养中是不可逆的。支持的平板凝胶电泳谱提供了暴露于CH3SH导致胶原代谢改变和I型前胶原堆积的证据。此外，CH3SH对细胞DNA合成的抑制作用在24~26h的高峰期达44.1%。这是一种真正的抑制，而不是最大DNA合成峰值的移动，因为控制系统和测试系统的时间-过程曲线的形状和位置非常相似。用[14C]-Pro进行的Pro转运研究表明，在CH3SH作用24到30小时的培养中，Pro转运减少了40%到50%。显着的是，即使暴露在6.7 ng CH3SH/ml的孵化气氛中15分钟，也会抑制大约24%的脯氨酸运输。这表明，即使是短暂暴露在低浓度的CH3SH中，也会对Pro的运输产生显著的不利影响。硫醇对组织切片的荧光染色表明，该试剂增加了染有活性染料的细胞的数量。
Methyl mercaptan (CH3SH) is a malodorous compound whose levels are elevated in mouth and crevicular air of individuals with active periodontal disease. Since it may play a role in the disease process, its effects were evaluated using human gingival fibroblast cultures and viable porcine unkeratinized oral mucosal tissue sections. Results showed that the protein content of CH3SH-exposed cell cultures pulsed with [14C]-labelled glycine and proline was decreased by approximately 25%. Furthermore, this deleterious effect was irreversible in test cultures subsequently incubated for 24 h in a control 95% air/5% CO2 mercaptan-free environment. The supporting slab-gel electrophoresis profiles yielded evidence that exposure to CH3SH caused an alteration in collagen metabolism and a pooling of Type I procollagens. In addition, DNA synthesis was suppressed in CH3SH-exposed cultures by 44.1% at the 24 to 26 h peak of DNA synthesis. This is a true inhibition and not a shift in peak of maximum DNA synthesis as the shape and location of time-course curves of control and test systems is very similar. Proline transport study using [14C]-proline indicated a reduction in proline transport in the range of 40 to 50% in cultures exposed for 24 to 30 h to CH3SH. Significantly even 15 min exposure to 6.7 ng CH3SH/ml of incubating atmosphere suppressed proline transport by approximately 24%. This indicates that even brief exposure to low concentrations of CH3SH has a significant adverse effect on proline transport. Fluorescent staining of tissue sections exposed to mercaptan indicated that the agent elevated the number of cells stained with vital dye.(ABSTRACT TRUNCATED AT 250 WORDS)
表皮生长因子对人成纤维细胞合成活性的影响。
DOI：
10.1016/0304-4165(80)90080-x
发表时间：
1980
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Huey,J;Narayanan,AS;Jones,K;Page,RC
通讯作者：
Page,RC