Long-term durable repaired cartilage induced by SOX9 in situ with bone marrow-derived mesenchymal stem cells.
Long-term durable repaired cartilage induced by SOX9 in situ with bone marrow-derived mesenchymal stem cells.
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DOI：
10.7150/ijms.52510
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发表时间：
2021
影响因子：
3.6
通讯作者：
Chu CQ
中科院分区：
文献类型：
--
作者：
Zhang X;Wu S;Zhu Y;Chu CQ
背景：微骨折术是软骨修复的常用方法，但常产生劣质纤维软骨。我们以前报道过，一个超正电荷的SOX 9（scSOX 9）促进透明样软骨再生诱导骨髓间充质干细胞分化成软骨细胞在体内。在这里，我们通过评估修复软骨的生物力学特性来检查由scSOX 9微骨折诱导的软骨修复的长期功效。方法：在新西兰雌兔右侧股骨髁间沟造成软骨缺损，行微骨折术。scS 0X 9蛋白质在并入胶原蛋白膜中的微骨折部位处施用。结果如下：在第12周和第24周，scSOX 9处理诱导透明样软骨，而单独的胶原膜诱导纤维软骨，并且突变体scSOX 9-A76 E不良地诱导软骨修复。scSOX 9处理组的软骨基质显示出高度富集的蛋白聚糖含量。与修复软骨的组织学特征和厚度一致，scSOX 9诱导的软骨的力学性能也与正常软骨相似。总结：这项长期体内研究表明，与微骨折结合，scSOX 9能够诱导具有长期持久的透明软骨特征的修复组织。这项技术有可能转化为软骨修复的临床应用，以防止骨关节炎的进展。
Background: Microfracture is a common procedure for cartilage repair, but it often produces inferior fibrocartilage. We previously reported that a super positively charged SOX9 (scSOX9) promoted hyaline-like cartilage regeneration by inducing bone marrow derived mesenchymal stem cell differentiation into chondrocytes in vivo. Here we examined the long-term efficacy of cartilage repair induced by microfracture with scSOX9 by assessing the biomechanical property of the repaired cartilage. Methods: A cartilage defect was created at the right femoral trochlear groove in New Zealand female rabbits and microfracture was performed. The scSOX9 protein was administered at the site of microfracture incorporated in a collagen membrane. Results: At 12 and 24 weeks, scSOX9 treatment induced hyaline-like cartilage while collagen-membrane alone induced fibrocartilage and mutant scSOX9-A76E poorly induced cartilage repair. The cartilage matrix in scSOX9-treated group showed highly enriched proteoglycan content. Consistent with the histological feature and the thickness of the repaired cartilage, the mechanical property of scSOX9-induced cartilage was also similar to that of normal cartilage. Conclusion: This long-term in vivo study demonstrated that in combination with microfracture, scSOX9 was able to induce reparative tissue with features of hyaline cartilage which was durable in long-term. This technology has the potential to translate into clinical use for cartilage repair to prevent progression to osteoarthritis.
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影响因子：
4.6
作者：
Fellows CR;Williams R;Davies IR;Gohil K;Baird DM;Fairclough J;Rooney P;Archer CW;Khan IM
通讯作者：
Khan IM
影响因子：
4.5
作者：
Leung VY;Gao B;Leung KK;Melhado IG;Wynn SL;Au TY;Dung NW;Lau JY;Mak AC;Chan D;Cheah KS
通讯作者：
Cheah KS
影响因子：
7
作者：
Tew, SR;Li, Y;Hardingham, TE
通讯作者：
Hardingham, TE
影响因子：
--
作者：
Li, Y;Tew, SR;Hawkins, RE
通讯作者：
Hawkins, RE
影响因子：
7
作者：
Sim, S.;Chevrier, A.;Buschmann, M. D.
通讯作者：
Buschmann, M. D.