Development of horizontal intrinsic connections in cat striate cortex-猫眼课题宝

猫纹状皮层水平内在连接的发展

DOI：

10.1007/bf00236865

发表时间：

2004

期刊：

Experimental Brain Research

影响因子：

通讯作者：

中科院分区：

医学4区

文献类型：

作者：

H. J. Luhmann;L. Millán;Wolf Singer

将辣根过氧化物酶与麦胚凝集素（WGA-HRP）结合，在猫纹状皮质的浅层内注射，显示出特征性的斑片状染色模式。斑块由标记的神经元和轴突分支的密集堆积组成。我们已经研究了切线组织和发展的内在皮质连接使用平面安装准备区17。斑块的直径为200 ~ 400 μm，中心距为400 ~ 800 μm，斑块在前后方向的扩展程度大于在内外方向的扩展程度。这些水平片状连接的表达与年龄和经验有关。从10天到6周龄，斑片旺盛，有时融合成从注射部位放射状延伸的珠状带。从6周开始，斑块的数量和切线扩散减少到一行或两行孤立的簇。长期的双眼剥夺几乎完全破坏了这种内在联系模式。我们从这些结果中推断，有一个先天的模式，离散的水平连接在纹状皮质，这是由视觉经验形成的，并需要轮廓视觉的维护。

SummaryIntracortical injections of horseradish peroxidase conjugated with wheat-germ agglutinin (WGA-HRP) reveal a characteristic patchy staining pattern within the superficial layers of cat striate cortex. The patches consist of a dense accumulation of labeled neurons and axonal arborizations. We have investigated the tangential organization and the development of these intrinsic cortical connections by using a flat-mount preparation of area 17. The diameter of the patches varied from 200 to 400 μm, the center-to-center distance ranged from 400 to 800 μm, and the spread of patches extended further in the anterior-posterior than in the medial-lateral direction. The expression of these horizontal patchy connections is age- and experience-dependent. From ten days to six weeks of age patches are exuberant and on occasion fuse to beaded bands extending radially from the injection site. From 6 weeks onwards the number and the tangential spread of the patches decreases to one or two rows of isolated clusters. Long-term binocular deprivation disrupts this pattern of intrinsic connections nearly completely. We infer from these results that there is an inborn pattern of discrete horizontal connections in striate cortex which is shaped by visual experience and requires contour vision for its maintenance.

Intrinsic projections within visual cortex: evidence for orientation-specific local connections.