Cell origin, volume and arrangement are drivers of articular cartilage formation, morphogenesis and response to injury in mouse limbs.
Cell origin, volume and arrangement are drivers of articular cartilage formation, morphogenesis and response to injury in mouse limbs.
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DOI：
10.1016/j.ydbio.2017.04.006
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发表时间：
2017-06-01
影响因子：
2.7
通讯作者：
Pacifici M
中科院分区：
文献类型：
--
作者：
Decker RS;Um HB;Dyment NA;Cottingham N;Usami Y;Enomoto-Iwamoto M;Kronenberg MS;Maye P;Rowe DW;Koyama E;Pacifici M
肢体滑膜关节由不同的组织组成，但目前尚不清楚哪些祖细胞产生这些组织以及关节软骨如何获得其功能性出生后组织，其特征在于软骨细胞柱，区域特异性细胞体积和各向异性基质。使用新的Gdf 5CreERT 2（Gdf 5-CE），Prg 4-CE和Dkk 3-CE小鼠交配R26-Confetti或单色报告，我们发现，膝关节祖细胞产生小的非迁移后代和不同的局部组织在产前和产后的时间。体视学成像和定量表明，青少年-成人胫骨关节软骨中存在的柱由非子代、部分重叠的谱系细胞组成，可能反映了细胞重排和堆叠。细胞体积的区域特异性增加是组织增厚的主要驱动因素，而细胞增殖或死亡起次要作用。二次谐波产生与2-光子显微镜显示，胶原蛋白基质从各向同性和分散在年轻的阶段是各向异性和对齐沿着细胞堆叠在成年人。在产前或幼年阶段的祖细胞追踪显示，关节损伤引起滑膜Prg 4+和CD 44 +/P75+细胞的大量和快速增加，其中一些填充损伤部位，而邻近的软骨细胞似乎没有反应。我们的数据表明，局部细胞群产生不同的关节组织，关节软骨生长和带状组织主要是由细胞体积扩张和地形细胞重排。滑膜Prg 4+谱系祖细胞对急性损伤有灵敏的反应，并可能代表关节组织修复的先驱。
Limb synovial joints are composed of distinct tissues, but it is unclear which progenitors produce those tissues and how articular cartilage acquires its functional postnatal organization characterized by chondrocyte columns, zone-specific cell volumes and anisotropic matrix. Using novel Gdf5CreERT2 (Gdf5-CE), Prg4-CE and Dkk3-CE mice mated to R26-Confetti or single-color reporters, we found that knee joint progenitors produced small non-migratory progenies and distinct local tissues over prenatal and postnatal time. Stereological imaging and quantification indicated that the columns present in juvenile-adult tibial articular cartilage consisted of non-daughter, partially overlapping lineage cells, likely reflecting cell rearrangement and stacking. Zone-specific increases in cell volume were major drivers of tissue thickening, while cell proliferation or death played minor roles. Second harmonic generation with 2-photon microscopy showed that the collagen matrix went from being isotropic and scattered at young stages to being anisotropic and aligned along the cell stacks in adults. Progenitor tracing at prenatal or juvenile stages showed that joint injury provoked a massive and rapid increase in synovial Prg4+ and CD44+/P75+ cells some of which filling the injury site, while neighboring chondrocytes appeared unresponsive. Our data indicate that local cell populations produce distinct joint tissues and that articular cartilage growth and zonal organization are mainly brought about by cell volume expansion and topographical cell rearrangement. Synovial Prg4+ lineage progenitors are exquisitely responsive to acute injury and may represent pioneers in joint tissue repair.
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影响因子：
2.4
作者：
Ito, MM;Kida, MY
通讯作者：
Kida, MY
慢循环细胞在附后软骨中的分布以及β-catenin信号在生长板中维持的需求。
影响因子：
2.8
作者：
Candela, Maria Elena;Cantley, Leslie;Yasuaha, Rika;Iwamoto, Masahiro;Pacifici, Maurizio;Enomoto-Iwamoto, Motomi
通讯作者：
Enomoto-Iwamoto, Motomi
DOI：
10.1126/science.1245075
发表时间：
2015-05-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ginzberg MB;Kafri R;Kirschner M
通讯作者：
Kirschner M
影响因子：
7
作者：
Julkunen, P.;Harjula, T.;Helminen, H. J.
通讯作者：
Helminen, H. J.
影响因子：
3.1
作者：
Gannon, A. R.;Nagel, T.;Kelly, D. J.
通讯作者：
Kelly, D. J.