Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in Arabidopsis.
Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in Arabidopsis.
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DOI：
10.1186/1471-2229-11-84
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发表时间：
2011-05-16
影响因子：
5.3
通讯作者：
Perez-Amador MA
中科院分区：
文献类型：
--
作者：
Carbonell-Bejerano P;Urbez C;Granell A;Carbonell J;Perez-Amador MA
胚珠寿命是决定坐果和产生种子能力的重要因素。一旦胚珠衰老，对赤霉素（GA）的反应能力就会丧失。我们的目的是阐明乙烯是否在控制拟南芥胚珠衰老和坐果反应中发挥作用。乙烯反应抑制剂、硫代硫酸银 (STS) 和 1-甲基环丙烯 (1-MCP) 能够延缓雌蕊对 GA3 反应的丧失。此外，乙烯不敏感突变体ein2-5和ein3-1表现出雌蕊反应延迟丧失，如在用STS和1-MCP处理的植物中一样，而组成型突变体ctr1-1表现出反应过早丧失。对乙烯生物合成基因表达的分析表明，乙烯是在胚珠衰老开始时在胚珠中合成的，而转录荟萃分析也支持在胚珠衰老建立时激活的乙烯依赖性衰老。最后，SAG12:GUS 报告系被证明可用于监测胚珠衰老并直接证明乙烯特异性调节胚珠衰老。我们已经证明乙烯参与胚珠寿命的控制和雌蕊/果实命运的决定。我们的数据支持拟南芥坐果期间胚珠在调节 GA 反应中的作用。讨论了将胚珠衰老的乙烯调节与 GA3 诱导的坐果反应联系起来的可能机制。
Ovule lifespan is an important factor in determining the ability to set fruits and produce seeds. Once ovule senescence is established, fruit set capacity in response to gibberellins (GAs) is lost. We aimed to elucidate whether ethylene plays a role in controlling ovule senescence and the fruit set response in Arabidopsis. Ethylene response inhibitors, silver thiosulphate (STS) and 1-methylcyclopropene (1-MCP), were able to delay the loss of pistil response to GA3. In addition, ethylene insensitive mutants ein2-5 and ein3-1 showed delayed loss of pistil response, as in plants treated with STS and 1-MCP, while constitutive mutant ctr1-1 displayed premature loss of response. The analysis of the expression of ethylene biosynthesis genes suggests that ethylene is synthesised in ovules at the onset of ovule senescence, while a transcriptional meta-analysis also supports an activated ethylene-dependent senescence upon the establishment of ovule senescence. Finally, a SAG12:GUS reporter line proved useful to monitor ovule senescence and to directly demonstrate that ethylene specifically modulates ovule senescence. We have shown that ethylene is involved in both the control of the ovule lifespan and the determination of the pistil/fruit fate. Our data support a role of the ovule in modulating the GA response during fruit set in Arabidopsis. A possible mechanism that links the ethylene modulation of the ovule senescence and the GA3-induced fruit set response is discussed.
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