Impacts of methamidophos on the biochemical, catabolic, and genetic characteristics of soil microbial communities
Impacts of methamidophos on the biochemical, catabolic, and genetic characteristics of soil microbial communities
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甲胺磷对土壤微生物群落生化、分解代谢和遗传特征的影响
DOI：
10.1016/j.soilbio.2007.10.012
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发表时间：
2008-03
影响因子：
9.7
通讯作者：
中科院分区：
文献类型：
--
作者：
甲胺磷是一种高毒性有机磷农药，对土壤微生物有显著影响。然而，这种影响的程度尚不清楚。采用微生物生物量、磷脂脂肪酸（PLFAs）、群落水平分解代谢谱（CLCPs）和扩增核糖体DNA限制性分析（ARP-RFLP）等多相分析方法，研究了低、高浓度甲胺磷输入对土壤微生物群落结构、分解代谢活性和细菌群落遗传多样性的影响。结果表明，高浓度甲胺磷处理显著降低了土壤微生物总生物量碳（Cmic）和真菌生物量，但增加了革兰氏阴性菌的数量，对革兰氏阳性菌的数量影响不显著。有趣的是，CLCPs模式表明，高甲胺磷投入也显着提高革兰氏阴性菌的分解代谢活性。化学胁迫下，细菌群落的遗传多样性降低。此外，微生物参数的变化在低投入下的甲胺磷比高投入的甲胺磷显着，这表明甲胺磷对微生物群落的剂量效应。我们的研究结果提供了第一个证据表明，甲胺磷差异影响土壤微生物群落的组成部分，通过抑制真菌的生长，但提高生物量和革兰氏阴性细菌的分解代谢活性。
Methamidophos is an organophosphate pesticide with high toxicity and may significantly affect soil microbes. However, the magnitude of this effect is unclear. We examined the effect of low and high inputs of methamidophos on the structure of the soil microbial community, and the catabolic activity and the genetic diversity of the bacterial community using the polyphasic approaches of microbial biomass, phospholipid fatty acids (PLFAs), community-level catabolic profiles (CLCPs), and amplified ribosomal DNA restriction analysis (ARDRA) patterns. Our results indicated that high methamidophos inputs significantly reduced total microbial biomass carbon (Cmic) and fungal biomass, but increased Gram-negative bacteria with no significant effects on the Gram-positive bacteria. Interestingly, CLCPs patterns showed that high methamidophos inputs also significantly improved the catabolic activity of Gram-negative bacteria. The ARDRA pattern showed that the genetic diversity of the bacterial community decreased under chemical stress. Furthermore, changes in the microbial parameters examined were less significant under low inputs than high inputs of methamidophos, suggesting a dosage effect of methamidophos on the microbial community. Our results provide the first evidence that methamidophos differentially affected components of the soil microbial community through inhibiting fungal growth but enhancing the biomass and catabolic activity of Gram-negative bacteria.
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影响因子：
4.4
作者：
Nakatsu, CH;Carmosini, N;Konopka, A
通讯作者：
Konopka, A
影响因子：
2.6
作者：
D. White;R. Findlay
通讯作者：
D. White;R. Findlay
影响因子：
4.8
作者：
Braun, B;Böckelmann, U;Szewzyk, U
通讯作者：
Szewzyk, U
影响因子：
4.7
作者：
S. Yu;S. Nguyen
通讯作者：
S. Yu;S. Nguyen
影响因子：
4.4
作者：
Zhou, JZ;Bruns, MA;Tiedje, JM
通讯作者：
Tiedje, JM