A Genome Wide Association Study of arabinoxylan content in 2-row spring barley grain.
A Genome Wide Association Study of arabinoxylan content in 2-row spring barley grain.
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DOI：
10.1371/journal.pone.0182537
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Burton RA
中科院分区：
文献类型：
--
作者：
Hassan AS;Houston K;Lahnstein J;Shirley N;Schwerdt JG;Gidley MJ;Waugh R;Little A;Burton RA
在大麦胚乳中，阿拉伯木聚糖（AX）是第二丰富的细胞壁多糖，并且在小麦中，它是谷粒的淀粉质胚乳壁中最丰富的多糖。AX是谷物膳食纤维含量的主要贡献者之一，提供多种健康益处，包括降低胆固醇和葡萄糖的作用以及抗氧化活性。由于其复杂的结构特征，AX也可能影响大麦籽粒在麦芽和酿造中的下游应用。利用高压液相色谱法（HPLC），我们定量分析了128个春二棱大麦品种成熟籽粒中AX的含量。含量范围为~ 5.2 μg/g至~ 9 μg/g。我们将这些数据用于全基因组关联研究（GWAS），该研究揭示了与谷物AX水平相关的三个显著的数量性状位点（QTL），其通过了错误发现阈值（FDR）并且位于七条大麦染色体中的两条上。对可能参与AX生物合成或周转的基因的QTL潜在区域进行扫描，并鉴定了强候选物，包括来自GT 43和GT 61家族的糖基转移酶和来自GH 10家族的糖苷水解酶。基于蛋白质翻译构建了所选基因家族的系统发育树，并用于研究大麦候选基因与其他物种的关系。我们的数据重申了现有基因的作用，认为有助于AX含量，并确定新的QTL（和候选基因与他们相关）可能影响AX含量的大麦籽粒。这项工作的一个潜在成果是在育种计划中部署高度相关的单核苷酸多态性标记，以指导大麦籽粒中AX丰度的修饰。
In barley endosperm arabinoxylan (AX) is the second most abundant cell wall polysaccharide and in wheat it is the most abundant polysaccharide in the starchy endosperm walls of the grain. AX is one of the main contributors to grain dietary fibre content providing several health benefits including cholesterol and glucose lowering effects, and antioxidant activities. Due to its complex structural features, AX might also affect the downstream applications of barley grain in malting and brewing. Using a high pressure liquid chromatography (HPLC) method we quantified AX amounts in mature grain in 128 spring 2-row barley accessions. Amounts ranged from ~ 5.2 μg/g to ~ 9 μg/g. We used this data for a Genome Wide Association Study (GWAS) that revealed three significant quantitative trait loci (QTL) associated with grain AX levels which passed a false discovery threshold (FDR) and are located on two of the seven barley chromosomes. Regions underlying the QTLs were scanned for genes likely to be involved in AX biosynthesis or turnover, and strong candidates, including glycosyltransferases from the GT43 and GT61 families and glycoside hydrolases from the GH10 family, were identified. Phylogenetic trees of selected gene families were built based on protein translations and were used to examine the relationship of the barley candidate genes to those in other species. Our data reaffirms the roles of existing genes thought to contribute to AX content, and identifies novel QTL (and candidate genes associated with them) potentially influencing the AX content of barley grain. One potential outcome of this work is the deployment of highly associated single nucleotide polymorphisms markers in breeding programs to guide the modification of AX abundance in barley grain.
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影响因子：
11.2
作者：
Höije, A;Gröndahl, M;Gatenholm, P
通讯作者：
Gatenholm, P
DOI：
10.1073/pnas.1202079109
发表时间：
2012-10-16
影响因子：
11.1
作者：
Chiniquy, Dawn;Sharma, Vaishali;Ronald, Pamela C.
通讯作者：
Ronald, Pamela C.
影响因子：
3.4
作者：
Chen, Chun-Chi;Huang, Jian-Wen;Guo, Rey-Ting
通讯作者：
Guo, Rey-Ting
影响因子：
7.2
作者：
Frankova, Lenka;Fry, Stephen C.
通讯作者：
Fry, Stephen C.
影响因子：
5.6
作者：
Burton RA;Fincher GB
通讯作者：
Fincher GB